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The effect of folic acid supplementation on dna biomarkers of colorectal cancer risk (uracil misincorporation, global and gene-specific dna hypomethylation) : a randomised intervention study

O'Reilly, S. L., McGlynn, A. P., Wasson, G. R., Bradbury, I., Mc Nulty, H., Strain, S. S., Weir, D. G., Mahmud, N., McKelvey-Martin, V., Downes, C. S. and Scott, J. M. 2007, The effect of folic acid supplementation on dna biomarkers of colorectal cancer risk (uracil misincorporation, global and gene-specific dna hypomethylation) : a randomised intervention study, in NSNZ & NSA 2006 : Proceedings of the Joint Annual Scientific Meeting of the Nutrition Society of New Zealand & the Nutrition Society of Australia, HEC Press, Melbourne, Vic., pp. S39-S39.

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Title The effect of folic acid supplementation on dna biomarkers of colorectal cancer risk (uracil misincorporation, global and gene-specific dna hypomethylation) : a randomised intervention study
Author(s) O'Reilly, S. L.ORCID iD for O'Reilly, S. L. orcid.org/0000-0003-3547-6634
McGlynn, A. P.
Wasson, G. R.
Bradbury, I.
Mc Nulty, H.
Strain, S. S.
Weir, D. G.
Mahmud, N.
McKelvey-Martin, V.
Downes, C. S.
Scott, J. M.
Conference name Nutrition Society of Australia. Scientific Meeting (31st :2007 : Auckland, New Zealand)
Conference location Auckland, New Zealand
Conference dates 5-7 Dec. 2007
Title of proceedings NSNZ & NSA 2006 : Proceedings of the Joint Annual Scientific Meeting of the Nutrition Society of New Zealand & the Nutrition Society of Australia
Publication date 2007
Start page S39
End page S39
Total pages 1
Publisher HEC Press
Place of publication Melbourne, Vic.
Keyword(s) folic acid
supplementation
colonic polyps
colorectal cancer
folate
Summary Background - Alterations in folate status are associated with colorectal carcinogenesis. Folate’s role has been postulated to be either via prevention of changes in DNA methylation or uracil misincorporation.
Objective - To investigate the effect of folic acid supplementation on colonocyte folate status and DNA biomarkers.
Design - Twenty individuals harbouring colonic adenomas were randomised to receive folic acid (600 g daily) or placebo for 6 months post polypectomy. Systemic and colonocyte folate status was determined at baseline and following the intervention. Modified Comet assays were used to determine uracil misincorporation and global DNA hypomethylation at the site adjacent to the polyp and a site distal to the polyp.
Outcomes - Supplementation resulted in increased colonocyte folate, which approached significance, at the site adjacent to the polyp (P= 0.06) but not distal to the polyp (P= 0.36); correspondingly there was a reduction in uracil misincorporation at the site adjacent to the polyp (P = 0.02) and the distal site showed no such trend (P= 0.39). There were no significant changes in global DNA hypomethylation at either site post-intervention.
Conclusions - Folic acid supplementation resulted in increased colonocyte folate and decreased uracil misincorporation at the site of the adenoma but not distal to the adenoma. This supports the hypothesis that localised areas of folate deficiency may exist in human colonic mucosa which respond to folic acid supplementation through increasing colonocyte folate and improving folate-related DNA biomarkers of cancer risk.


ISSN 0964-7058
1440-6047
Language eng
Field of Research 111104 Public Nutrition Intervention
Socio Economic Objective 920102 Cancer and Related Disorders
HERDC Research category E3.1 Extract of paper
HERDC collection year 2007
Copyright notice ©2001-2007, HEC PRESS
Persistent URL http://hdl.handle.net/10536/DRO/DU:30031264

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Created: Fri, 19 Nov 2010, 13:35:58 EST by Sharleen O'reilly

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