High-performance liquid chromatography with post-column 2,2′-diphenyl-1-picrylhydrazyl radical scavenging assay : methodological considerations and application to complex samples

McDermott, Geoffrey P., Noonan, Laura K., Mnatsakanyan, Mariam, Shalliker, R. Andrew, Conlan, Xavier A., Barnett, Neil W. and Francis, Paul S. 2010, High-performance liquid chromatography with post-column 2,2′-diphenyl-1-picrylhydrazyl radical scavenging assay : methodological considerations and application to complex samples, Analytica chimica acta, vol. 675, no. 1, pp. 76-82.

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Title High-performance liquid chromatography with post-column 2,2′-diphenyl-1-picrylhydrazyl radical scavenging assay : methodological considerations and application to complex samples
Author(s) McDermott, Geoffrey P.
Noonan, Laura K.
Mnatsakanyan, Mariam
Shalliker, R. Andrew
Conlan, Xavier A.
Barnett, Neil W.
Francis, Paul S.
Journal name Analytica chimica acta
Volume number 675
Issue number 1
Start page 76
End page 82
Total pages 7
Publisher Elsevier B.V.
Place of publication Amsterdam, The Netherlands
Publication date 2010-08-18
ISSN 0003-2670
1873-4324
Keyword(s) 2,2´-Diphenyl-1-picrylhydrazyl
radical scavenging assay
antioxidants
high-performance liquid chromatography
green tea
thyme
Summary An improved post-column 2,2´-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging assay for the screening of antioxidants in complex matrices was developed. Experimental parameters believed to be influential to DPPH• response were studied in a univariate approach. Optimum conditions were found to be: 5×10−5M DPPH• reagent prepared in a 75% methanol: 25% 40mM citric acid–sodium citrate buffer (pH 6) solution, degassed with nitrogen; reaction coil of 2m×0.25mm i.d. PEEK tubing; detection at 521 nm; analysis at room temperature. The analytical utility of this protocol was evaluated by screening for antioxidants in thyme and green tea, in comparison with two commonly employed methodologies.
Notes Available online 23 July 2010
Language eng
Field of Research 030108 Separation Science
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2010
Related work DU:30042598
Copyright notice ©2010, Elsevier B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30031280

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