Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei Stewart, Michael J., Stewart, Praphaporn, Soonklang, Nantawan, Linthong, Vichai, Hanna, Peter J., Duan, Wei and Sobhon, Prasert 2010, Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei, Tissue and cell, vol. 42, no. 3, pp. 137-150. Attached Files Name Description MIMEType Size Downloads Title Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei Formatted title Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei Author(s) Stewart, Michael J. Stewart, Praphaporn Soonklang, Nantawan Linthong, Vichai Hanna, Peter J. Duan, Wei Sobhon, Prasert Journal name Tissue and cell Volume number 42 Issue number 3 Start page 137 End page 150 Total pages 14 Publisher Churchill Livingstone Place of publication United Kingdom Publication date 2010-06 ISSN 0040-8166 1532-3072 Keyword(s) Portunus pelagicus spermatogenesis spermatozoa ultrastructure basic nuclear proteins Summary Spermatogenesis in the blue swimming crab, Portunus pelagicus, is described by light and electron microscopy. The testis is composed of anterior (AT) and posterior (PT) lobes, that are partitioned into lobules by connective tissue trabecula, and further divided into zones (germinal, transformation and evacuation), each with various stages of cellular differentiation. The vas deferens is classified into three distinct regions: anterior (AVD), median (MVD), and posterior (PVD), on the presence of spermatophores and two secretions, termed substance I and II. Based on the degree and patterns of heterochromatin, spermatogenesis is classified into 13 stages: two spermatogonia (SgA and SgB), six primary spermatocytes (leptotene, zygotene, pachytene, diplotene, diakinesis, and metaphase), a secondary spermatocyte (SSc), three spermatids (St 1–3), and a mature spermatozoon. Spermatid stages are differentiated by chromatin decondensation and the formation of an acrosomal complex, which is unique to brachyurans. Mature spermatozoa are aflagellated, and have a nuclear projection and a spherical acrosome. AUT-PAGE and Western blots show that, during chromatin decondensation, there is a reduction of most histones, with only small amounts of H2B and H3 remaining in mature spermatozoa. Language eng Field of Research 070401 Aquaculture Socio Economic Objective 830299 Fisheries- Wild Caught not elsewhere classified HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article HERDC collection year 2010 Copyright notice ©2010, Elsevier Ltd. Persistent URL http://hdl.handle.net/10536/DRO/DU:30031456 Document type: Journal Article Collections: School of Medicine School of Life and Environmental Sciences   Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Mon, 06 Dec 2010, 13:09:47 EST Tue, 07 Dec 2010, 10:48:11 EST Tue, 07 Dec 2010, 10:49:19 EST Wed, 08 Dec 2010, 07:33:04 EST Fri, 10 Jun 2011, 08:58:58 EST Fri, 10 Jun 2011, 09:00:11 EST Wed, 01 Feb 2012, 15:11:42 EST Wed, 01 Feb 2012, 15:12:40 EST Filtered Full Citation counts:   Cited 27 times in TR Web of Science Cited 27 times in Scopus Search Google Scholar Access Statistics: 523 Abstract Views, 1 File Downloads  -  Detailed Statistics Created: Mon, 06 Dec 2010, 13:09:44 EST by Penny Andrews

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