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Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei

Stewart, Michael J., Stewart, Praphaporn, Soonklang, Nantawan, Linthong, Vichai, Hanna, Peter J., Duan, Wei and Sobhon, Prasert 2010, Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei, Tissue and cell, vol. 42, no. 3, pp. 137-150.

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Title Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei
Formatted title Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei
Author(s) Stewart, Michael J.
Stewart, Praphaporn
Soonklang, Nantawan
Linthong, Vichai
Hanna, Peter J.
Duan, Wei
Sobhon, Prasert
Journal name Tissue and cell
Volume number 42
Issue number 3
Start page 137
End page 150
Publisher Churchill Livingstone
Place of publication United Kingdom
Publication date 2010-06
ISSN 0040-8166
1532-3072
Keyword(s) Portunus pelagicus
spermatogenesis
spermatozoa
ultrastructure
basic nuclear proteins
Summary Spermatogenesis in the blue swimming crab, Portunus pelagicus, is described by light and electron microscopy. The testis is composed of anterior (AT) and posterior (PT) lobes, that are partitioned into lobules by connective tissue trabecula, and further divided into zones (germinal, transformation and evacuation), each with various stages of cellular differentiation. The vas deferens is classified into three distinct regions: anterior (AVD), median (MVD), and posterior (PVD), on the presence of spermatophores and two secretions, termed substance I and II. Based on the degree and patterns of heterochromatin, spermatogenesis is classified into 13 stages: two spermatogonia (SgA and SgB), six primary spermatocytes (leptotene, zygotene, pachytene, diplotene, diakinesis, and metaphase), a secondary spermatocyte (SSc), three spermatids (St 1–3), and a mature spermatozoon. Spermatid stages are differentiated by chromatin decondensation and the formation of an acrosomal complex, which is unique to brachyurans. Mature spermatozoa are aflagellated, and have a nuclear projection and a spherical acrosome. AUT-PAGE and Western blots show that, during chromatin decondensation, there is a reduction of most histones, with only small amounts of H2B and H3 remaining in mature spermatozoa.
Language eng
Field of Research 070401 Aquaculture
Socio Economic Objective 830299 Fisheries- Wild Caught not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
HERDC collection year 2010
Copyright notice ©2010, Elsevier Ltd.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30031456

Document type: Journal Article
Collections: School of Medicine
School of Life and Environmental Sciences
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Created: Mon, 06 Dec 2010, 13:09:44 EST by Penny Andrews