Therapeutic effects of matrine on primary and metastatic breast cancer

Li, Hali, Tan, Gang, Jiang, Xian, Qiao, Haiquan, Pan, Shanghua, Jiang, Hongchi, Kanwar, Jagat R. and Sun, Xueying 2010, Therapeutic effects of matrine on primary and metastatic breast cancer, The American journal of Chinese medicine, vol. 38, no. 6, pp. 1115-1130.

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Title Therapeutic effects of matrine on primary and metastatic breast cancer
Author(s) Li, Hali
Tan, Gang
Jiang, Xian
Qiao, Haiquan
Pan, Shanghua
Jiang, Hongchi
Kanwar, Jagat R.
Sun, Xueying
Journal name The American journal of Chinese medicine
Volume number 38
Issue number 6
Start page 1115
End page 1130
Total pages 16
Publisher World Scientific Publishing Co. Pte. Ltd.
Place of publication Toh Tuck Link, Singapore
Publication date 2010-12
ISSN 0192-415X
1793-6853
Keyword(s) matrine
breast cancer
metastasis
apoptosis
proliferation
angiogenesis
Summary Matrine, one of the main components extracted from a traditional Chinese herb, Sophora flavescens Ait, has displayed anti-cancer activity in several types of cancer cells. This study aims to evaluate the therapeutic benefits of matrine on primary and metastatic breast cancer. Matrine inhibited the viability of and induced apoptosis in human MCF-7 and mouse 4T1 breast cancer cells in a dose-dependent manner in vitro as shown by MTT assay, flow cytometry and laser scanning confocal microscopy. Administration of matrine inhibited the growth of primary tumors and their metastases to lungs and livers, in a dose-dependent manner, in a highly metastatic model of 4T1 breast cancer established in syngeneic Balb/c mice. Tumors from matrine-treated mice had a smaller proliferation index, shown by immunostaining with an anti-Ki-67 antibody, a greater apoptosis index, shown by TUNEL-staining, and a less microvessel density, shown by immunostaining with an anti-CD31 A antibody, compared to the controls. Western blot analysis of tumoral homogenates indicated that matrine therapy reduced the ratio of Bcl-2/Bax, downregulated the expressions of VEGF and VEGFR-2, and increased the activation of caspase-3 and caspase-9. This study suggests matrine may be a potent agent, from a natural resource, for treating metastatic breast cancer because of its anti-apoptotic, anti-proliferative and anti-angiogenic activities.
Language eng
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2010
Persistent URL http://hdl.handle.net/10536/DRO/DU:30031507

Document type: Journal Article
Collection: Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)
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