Ingrowth of human mesenchymal stem cells into porous silk particle reinforced silk composite scaffolds : an in vitro study

Rockwood, Danielle N., Gil, Eun Seok, Park, Sang-Hyug, Kluge, Jonathan A., Grayson, Warren, Bhumiratana, Sarindr, Rajkhowa, Rangam, Wang, Xungai, Kim, Sung Jun, Vunjack-Novakovic, Gordana and Kaplan, David L. 2011, Ingrowth of human mesenchymal stem cells into porous silk particle reinforced silk composite scaffolds : an in vitro study, Acta biomaterialia, vol. 7, no. 1, pp. 144-151, doi: 10.1016/j.actbio.2010.07.020.

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Title Ingrowth of human mesenchymal stem cells into porous silk particle reinforced silk composite scaffolds : an in vitro study
Author(s) Rockwood, Danielle N.
Gil, Eun Seok
Park, Sang-Hyug
Kluge, Jonathan A.
Grayson, Warren
Bhumiratana, Sarindr
Rajkhowa, RangamORCID iD for Rajkhowa, Rangam
Wang, XungaiORCID iD for Wang, Xungai
Kim, Sung Jun
Vunjack-Novakovic, Gordana
Kaplan, David L.
Journal name Acta biomaterialia
Volume number 7
Issue number 1
Start page 144
End page 151
Total pages 8
Publisher Elsevier Ltd.
Place of publication Amsterdam, Netherlands
Publication date 2011-01
ISSN 1742-7061
Keyword(s) osteogenesis
human mesenchymal stem cells
matrix stiffness
fibre science
Summary Silk fibroin protein is biodegradable and biocompatible, exhibiting excellent mechanical properties for various biomedical applications. However, porous three-dimensional (3-D) silk fibroin scaffolds, or silk sponges, usually fall short in matching the initial mechanical requirements for bone tissue engineering. In the present study, silk sponge matrices were reinforced with silk microparticles to generate protein-protein composite scaffolds with desirable mechanical properties for in vitro osteogenic tissue formation. It was found that increasing the silk microparticle loading led to a substantial increase in the scaffold compressive modulus from 0.3 MPa (non-reinforced) to 1.9 MPa for 1:2 (matrix:particle) reinforcement loading by dry mass. Biochemical, gene expression, and histological assays were employed to study the possible effects of increasing composite scaffold stiffness, due to microparticle reinforcement, on in vitro osteogenic differentiation of human mesenchymal stem cells (hMSCs). Increasing silk microparticle loading increased the osteogenic capability of hMSCs in the presence of bone morphogenic protein-2 (BMP-2) and other osteogenic factors in static culture for up to 6 weeks. The calcium adsorption increased dramatically with increasing loading, as observed from biochemical assays, histological staining, and microcomputer tomography (μCT) analysis. Specifically, calcium content in the scaffolds increased by 0.57, 0.71, and 1.27 mg (per μg of DNA) from 3 to 6 weeks for matrix to particle dry mass loading ratios of 1:0, 1:1, and 1:2, respectively. In addition, μCT imaging revealed that at 6 weeks, bone volume fraction increased from 0.78% for non-reinforced to 7.1% and 6.7% for 1:1 and 1:2 loading, respectively. Our results support the hypothesis that scaffold stiffness may strongly influence the 3-D in vitro differentiation capabilities of hMSCs, providing a means to improve osteogenic outcomes.
Language eng
DOI 10.1016/j.actbio.2010.07.020
Field of Research 091012 Textile Technology
Socio Economic Objective 860403 Natural Fibres, Yarns and Fabrics
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2010, Acta Materialia Inc.
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Document type: Journal Article
Collections: Centre for Material and Fibre Innovation
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