Influence of age on expression of DNA repair genes in Arabidopsis

Osmond, Megan J and Kunz, Bernard A 2010, Influence of age on expression of DNA repair genes in Arabidopsis. In Thomas, Allison E. (ed), DNA damage repair, repair mechanisms and aging, Nova Science Publishers, Inc, Hauppauge, N.Y., pp.263-274.

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Title Influence of age on expression of DNA repair genes in Arabidopsis
Author(s) Osmond, Megan J
Kunz, Bernard A
Title of book DNA damage repair, repair mechanisms and aging
Editor(s) Thomas, Allison E.
Publication date 2010
Series DNA and RNA : Properties and Modifications, Functions and Interactions, Recombination and Applications
Chapter number 13
Total chapters 13
Start page 263
End page 274
Total pages 12
Publisher Nova Science Publishers, Inc
Place of Publication Hauppauge, N.Y.
Keyword(s) Arabidopsis thaliana,
DNA repair
Ultraviolet radiation
Summary Numerous studies suggest that ageing in mammals may be associated with a reduction in DNA repair, whereas little is known about the DNA repair capacity of plants as they age. In this study we examined the effects of ageing on the expression of genes thought to be involved in nucleotide excision repair (AtERCC1, AtGTF2H2, AtGTF2H5, AtXPB1, AtXPD, AtXPF) or translesion replication (AtPOLH, AtREV1, AtREV3, AtUBC2) of UV photoproducts in Arabidopsis (Arabidopsis thaliana). Two- or four-week old plants were unirradiated or treated with 254 nm ultraviolet (UV) radiation (0.75 or 1.5 kJm-2), incubated for 3 or 9 hr, and gene expression was analysed via quantitative PCR. With the exception of AtPOLH, transcript levels for all genes investigated were at least four-fold greater in unirradiated four-week old plants than unirradiated two-week old plants. Compared to unirradiated age-matched plants, two-week old plants generally showed no consistent change in transcript levels for either UV dose or post-irradiation incubation period. On the other hand, transcript levels in four-week old plants were increased over those in two-week old plants for the majority of genes by 9 hr post-irradiation with 0.75 or 1.5 kJm-2 UV. No other consistent responses were observed for UV treatment. Collectively, our results are consistent with the possibility that ageing may be associated with increased DNA repair capacity in plants.
ISBN 9781616689148
Language eng
Field of Research 060405 Gene Expression (incl Microarray and other genome-wide approaches)
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category B1 Book chapter
ERA Research output type B Book chapter
HERDC collection year 2010
Copyright notice ©2010, Nova Science Publishers, Inc
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Document type: Book Chapter
Collection: School of Life and Environmental Sciences
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