Sequence analysis of a salt tolerant metagenomic clone

Kapardar, Raj Kishor, Ranjan, Ravi, Puri, Munish and Sharma, Rakesh 2010, Sequence analysis of a salt tolerant metagenomic clone, Indian journal of microbiology, vol. 50, no. 2, pp. 212-215.

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Title Sequence analysis of a salt tolerant metagenomic clone
Author(s) Kapardar, Raj Kishor
Ranjan, Ravi
Puri, Munish
Sharma, Rakesh
Journal name Indian journal of microbiology
Volume number 50
Issue number 2
Start page 212
End page 215
Total pages 4
Publisher Springer (India) Private Ltd
Place of publication New Delhi, India
Publication date 2010-06
ISSN 0046-8991
0973-7715
Keyword(s) metagenomic
metagenome
salt tolerance
ClpS
Summary

Metagenome represent an unlimited resource for discovery of novel genes. Here we report, sequence analysis of a salt tolerant metagenomic clone (6B4) from a pond water metagenomic library. Clone 6B4 had an insert of 2254 bp with G+C composition of 64.06%. DNA sequence from 6B4 showed homology to DNA sequences from proteobacteria indicating origin of 6B4 metagenomic insert from a yet uncharacterized proteobacteria. Two encoded proteins from clone 6B4 showed match with ATP-dependent Clp protease adaptor protein (ClpS) and phasin, while two truncated encoded proteins showed match with poly-3-hydroxybutyrate synthase and permease. Clp complex is known to play a role in stress tolerance. Expression of ClpS from metagenomic clone is proposed to be responsible for salt tolerance of the metagenomic clone 6B4.

Language eng
Field of Research 100301 Biocatalysis and Enzyme Technology
Socio Economic Objective 850501 Biofuel (Biomass) Energy
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2010
Copyright notice ©2010, Association of Microbiologists of India
Persistent URL http://hdl.handle.net/10536/DRO/DU:30033608

Document type: Journal Article
Collection: Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)
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