Sequence analysis of a salt tolerant metagenomic clone

Kapardar, Raj Kishor, Ranjan, Ravi, Puri, Munish and Sharma, Rakesh 2010, Sequence analysis of a salt tolerant metagenomic clone, Indian journal of microbiology, vol. 50, no. 2, pp. 212-215, doi: 10.1007/s12088-010-0041-x.

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Title Sequence analysis of a salt tolerant metagenomic clone
Author(s) Kapardar, Raj Kishor
Ranjan, Ravi
Puri, MunishORCID iD for Puri, Munish
Sharma, Rakesh
Journal name Indian journal of microbiology
Volume number 50
Issue number 2
Start page 212
End page 215
Total pages 4
Publisher Springer (India) Private Ltd
Place of publication New Delhi, India
Publication date 2010-06
ISSN 0046-8991
Keyword(s) metagenomic
salt tolerance

Metagenome represent an unlimited resource for discovery of novel genes. Here we report, sequence analysis of a salt tolerant metagenomic clone (6B4) from a pond water metagenomic library. Clone 6B4 had an insert of 2254 bp with G+C composition of 64.06%. DNA sequence from 6B4 showed homology to DNA sequences from proteobacteria indicating origin of 6B4 metagenomic insert from a yet uncharacterized proteobacteria. Two encoded proteins from clone 6B4 showed match with ATP-dependent Clp protease adaptor protein (ClpS) and phasin, while two truncated encoded proteins showed match with poly-3-hydroxybutyrate synthase and permease. Clp complex is known to play a role in stress tolerance. Expression of ClpS from metagenomic clone is proposed to be responsible for salt tolerance of the metagenomic clone 6B4.

Language eng
DOI 10.1007/s12088-010-0041-x
Field of Research 100301 Biocatalysis and Enzyme Technology
Socio Economic Objective 850501 Biofuel (Biomass) Energy
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2010
Copyright notice ©2010, Association of Microbiologists of India
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Created: Wed, 23 Mar 2011, 10:13:15 EST by Munish Puri

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