Citrus peel influences the production of an extracellular naringinase by Staphylococcus xylosus MAK2 in a stirred tank reactor

Munish, Puri, Kaur, Aneet, Barrow, Colin J. and Singh, Ram Sarup 2011, Citrus peel influences the production of an extracellular naringinase by Staphylococcus xylosus MAK2 in a stirred tank reactor, Applied microbiology and biotechnology, vol. 89, no. 3, pp. 715-722.

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Title Citrus peel influences the production of an extracellular naringinase by Staphylococcus xylosus MAK2 in a stirred tank reactor
Formatted title Citrus peel influences the production of an extracellular naringinase by Staphylococcus xylosus MAK2 in a stirred tank reactor
Author(s) Munish, Puri
Kaur, Aneet
Barrow, Colin J.
Singh, Ram Sarup
Journal name Applied microbiology and biotechnology
Volume number 89
Issue number 3
Start page 715
End page 722
Total pages 8
Publisher Springer
Place of publication Heidelberg, Germany
Publication date 2011
ISSN 0175-7598
1432-0614
Keyword(s) citrus peel
rhamnosidase
naringin
bioreactor
flavonoid
Summary Staphylococcus xylosus MAK2, Gram-positive coccus, a nonpathogenic member of the coagulase-negative Staphylococcus family was isolated from soil and used to produce naringinase in a stirred tank reactor. An initial medium at pH 5.5 and a cultivation temperature of 30°C was found to be optimal for enzyme production. The addition of Ca+2 caused stimulation of enzyme activity. The effect of various physico-chemical parameters, such as pH, temperature, agitation, and inducer concentration was studied. The enzyme production was enhanced by the addition of citrus peel powder (CPP) in the optimized medium. A twofold increase in naringinase production was achieved using different technological combinations. The process optimization using technological combinations allowed rapid optimization of large number of variables, which significantly improved enzyme production in a 5-l reactor in 34 h. An increase in sugar concentration (15 gl-1) in the fermentation medium further increased naringinase production (8.9 IUml-1) in the bioreactor. Thus, availability of naringinase renders it attractive for potential biotechnological applications in citrus processing industry.
Notes Published online 5 October 2010
Language eng
Field of Research 100302 Bioprocessing, Bioproduction and Bioproducts
100301 Biocatalysis and Enzyme Technology
Socio Economic Objective 850501 - Biofuel (Biomass) Energy
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2011
Copyright notice ©2010, Springer-Verlag
Persistent URL http://hdl.handle.net/10536/DRO/DU:30033615

Document type: Journal Article
Collection: Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)
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Created: Wed, 23 Mar 2011, 11:06:55 EST by Munish Puri

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