Fermentative production of rhamnosidase from Staphylococcus xylosus MAK 2 in a bioreactor for bio-energy generation

Puri, Munish and Kaur, Aneet 2010, Fermentative production of rhamnosidase from Staphylococcus xylosus MAK 2 in a bioreactor for bio-energy generation, in GIM 2010 : Program handbook of the 11th International Symposium on the Genetics of Industrial Microorganism (GIM) 2010, The Conference, [Melbourne, Vic.], pp. 67-67.

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Title Fermentative production of rhamnosidase from Staphylococcus xylosus MAK 2 in a bioreactor for bio-energy generation
Author(s) Puri, Munish
Kaur, Aneet
Conference name International Symposium on the Genetics of Industrial Microorganism (11th : 2010 : Melbourne, Victoria)
Conference location Melbourne, Victoria
Conference dates 28 Jun.- 1 Jul. 2010
Title of proceedings GIM 2010 : Program handbook of the 11th International Symposium on the Genetics of Industrial Microorganism (GIM) 2010
Publication date 2010
Start page 67
End page 67
Publisher The Conference
Place of publication [Melbourne, Vic.]
Summary Increasing concern about the environment, food and feed shortages and hike in the price of petroleum have stimulated interest in new ways of producing biofuels. The interest is rapidly increasing towards converting agricultural wastes to commercially valuable products. Biofuels made from waste biomass can offer immediate and sustained greenhouse gas advantages. In this direction, we are focusing on Citrus processing waste, a byproduct of juice manufacture, which contains high amount of flavonoids and polysaccharides. There is a considerable industrial interest in the enzymatic transformation of flavonoids to hydrolysis products; that offers a pathway to bio-energy generation. Rhamnosidase of bacterial origin are very few and thus are potentially subject for research.

Staphylococcus xylosus, Gram positive cocci, a nonpathogenic member of CNS family, isolated from soil was used to produce α-L-rhamnosidase. This new strain, so far unknown for the production of α-L-Rhamnosidase, was identified and characterized as Staphyloccocus sp. through biochemical tests and 16S DNA sequence analysis. Effect of various medium and process parameters like pH, temperature, aeration and agitation rates and inducer concentration were studied. Further, the enzyme activity was enhanced by adding the inducer and divalent metal ion to the optimised fermentation medium. We have recovered important sugars “rhamnose” and “galacturonic acid” from the processed waste which would be utilized for ethanol production. This presentation will summarize current efforts to develop an enzymatic treatment which would facilitate the economical processing of citrus waste for bioenergy generation.
Notes Conference website : http://www.gim2010.org/
Language eng
Field of Research 100302 Bioprocessing, Bioproduction and Bioproducts
Socio Economic Objective 850501 Biofuel (Biomass) Energy
HERDC Research category E3 Extract of paper
HERDC collection year 2010
Persistent URL http://hdl.handle.net/10536/DRO/DU:30033623

Document type: Conference Paper
Collection: Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)
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