Molecular characterization and enzymatic hydrolysis of flavanoid extracted from citrus waste

Puri, Munish 2010, Molecular characterization and enzymatic hydrolysis of flavanoid extracted from citrus waste, in Abstract booklet of the 7th International Symposium of Industrial Microbiology and Biotechnology, The Conference, Melbourne, Victoria, pp. 34-34.

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Title Molecular characterization and enzymatic hydrolysis of flavanoid extracted from citrus waste
Author(s) Puri, Munish
Conference name International Symposium of Industrial Microbiology and Biotechnology (7th : 2010 : Melbourne, Victoria)
Conference location Melbourne, Victoria
Conference dates 1-3 Jul. 2010
Title of proceedings Abstract booklet of the 7th International Symposium of Industrial Microbiology and Biotechnology
Editor(s) Britz, Margaret
Publication date 2010
Start page 34
End page 34
Publisher The Conference
Place of publication Melbourne, Victoria
Summary The citrus fruit processing industry generates substantial quantities of waste rich in phenolic substances, which is a valuable natural source of polyphenols (flavonoids) such as naringin and its disposal is becoming a major problem. In the US alone, the juice processing of oranges and grapefruit generates over 5 Mt of citrus waste every year. In the case of India, about 2.15 Mt of citrus peel out of 6.28 Mt of citrus fruits are produced yearly from citrus juice processing. In case of Australia, about 15-40% of citrus peel waste is generated by processing of citrus fruit (0.85 Mt). Thus Isolation of functional compounds (mostly flavanoids) and their further processing can be of interest to the food and pharmaceutical industry. This peel is rich in naringin and may be used for rhamnose production by utilizing α-L-rhamnosidase (EC 3.2.1.40), an enzyme that catalyzes the cleavage of terminal rhamnosyl groups from naringin to yield prunin and rhamnose. We recently purified recombinant α-L-rhamnosidase from E. coli cells using immobilized metal-chelate affinity chromatography (IMAC) and used it for naringin hydrolysis. The purified enzyme established hydrolysis of naringin extracted from citrus peel and thus endorses its industrial applicability for producing rhamnose. Infrared (IR) spectroscopy confirmed molecular characteristics of naringin extracted from citrus peel waste.
Notes Abstract book website :
http://www.margaretbritz.com/Final%207th%20International%20Symposium%20on%20Industrial%20Micrbiology%20%20Biotechnology%20%20Program%2026%20June%202010.pdf
Language eng
Field of Research 100302 Bioprocessing, Bioproduction and Bioproducts
Socio Economic Objective 850501 - Biofuel (Biomass) Energy
HERDC Research category E3 Extract of paper
HERDC collection year 2010
Persistent URL http://hdl.handle.net/10536/DRO/DU:30033625

Document type: Conference Paper
Collection: Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)
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