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Immobilization of bacterial recombinant proteins

Puri, Munish 2007, Immobilization of bacterial recombinant proteins, in New horizons in biotechnology, Asiatech Publishers, New Delhi, India.

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Title Immobilization of bacterial recombinant proteins
Author(s) Puri, MunishORCID iD for Puri, Munish
Conference name International Conference on New Horizons in Biotechnology (2nd : 2007 : Trivandrum, India)
Conference location Trivandrum, India
Conference dates 26-29 Nov. 2007
Title of proceedings New horizons in biotechnology
Editor(s) Pandey, Ashok K.
Publication date 2007
Total pages vi, 456 p.
Publisher Asiatech Publishers
Place of publication New Delhi, India
Summary Recombinant α-L Rhamnosidase has several potential applications in citrus fruit juice processing industries. Immobilized recombinant α-L Rhamnosidase further provides an added advantage to this industrially important enzyme. Various techniques have been used to immobilize native rhamnosidase from fungal origin and applications were explored in great details by several workers. (Puri et al., 1996, 2000, 2001)

A recombinant rhamnosidase from a bacterial source was expressed in E.coli has been immobilized in calcium alginate beads (entrapment method). A batch bioreactor was created for the hydrolysis of naringin using immobilized recombinant α-L Rhamnosidase under shaking and stationary conditions and it was found to hydrolyze naringin effectively. The system was efficient to hydrolyze narigin under shaking conditions and was operationally stable up to 9 days. A high percent hydrolysis of naringin was achieved at pH 7.5 and 60˚C by immobilized rhamnosidase. Entrapped rhamnosidase was able to hydrolyze naringin content in kinnow juice repeatedly and this feature makes this technique economically suitable for debittering of fruit juices.
ISBN 9788187680192
Language eng
Field of Research 100302 Bioprocessing, Bioproduction and Bioproducts
Socio Economic Objective 860105 Nutraceuticals and Functional Foods
HERDC Research category E2.1 Full written paper - non-refereed / Abstract reviewed
HERDC collection year 2007
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Document type: Conference Paper
Collection: Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)
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Created: Wed, 23 Mar 2011, 15:36:59 EST by Munish Puri

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