Acetylene plasma polymerized surfaces for covalent immobilization of dense bioactive protein monolayers

Yin, Yongbai, Bilek, Marcela M. M., McKenzie, David R., Nosworthy, Neil J., Kondyurin, Alexey, Youssef, Hani, Byrom, Michael J. and Yang, Wenrong 2009, Acetylene plasma polymerized surfaces for covalent immobilization of dense bioactive protein monolayers, Surface and coatings technology, vol. 203, no. 10-11, pp. 1310-1316, doi: 10.1016/j.surfcoat.2008.10.035.

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Title Acetylene plasma polymerized surfaces for covalent immobilization of dense bioactive protein monolayers
Author(s) Yin, Yongbai
Bilek, Marcela M. M.
McKenzie, David R.
Nosworthy, Neil J.
Kondyurin, Alexey
Youssef, Hani
Byrom, Michael J.
Yang, WenrongORCID iD for Yang, Wenrong
Journal name Surface and coatings technology
Volume number 203
Issue number 10-11
Start page 1310
End page 1316
Total pages 7
Publisher Elsevier B. V.
Place of publication Amsterdam, The Netherlands
Publication date 2009-02-25
ISSN 0257-8972
Keyword(s) plasma polymerization
protein immobilization
covalent attachment
quartz crystal microbalance
Summary Smooth polymerized surfaces, suitable for biochemical and biomedical applications, were deposited using a modified plasma enhanced chemical vapour deposition method with acetylene as a reaction precursor. Horseradish peroxidase (HRP) activity assays showed that the protein immobilized on the plasma polymerized surfaces maintained its biological function for a much longer period of time compared to that on uncoated surfaces. The kinetics of HRP attachment to the plasma polymerized surfaces were analyzed using quartz crystal microbalance with dissipation analysis. Spectroscopic ellipsometry and attenuated total reflection Fourier transform infrared spectroscopy were used to determine the thickness and the quantity of the attached protein. The results showed that the plasma polymerized surfaces provided a high density of attachment sites to covalently immobilize a dense monolayer of proteins.
Language eng
DOI 10.1016/j.surfcoat.2008.10.035
Field of Research 100703 Nanobiotechnology
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2008, Elsevier B.V.
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Document type: Journal Article
Collection: Institute for Technology Research and Innovation
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