Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches

Klaassen, Marcel, Piersma, Theunis, Korthals, Harry, Dekinga, Anne and Dietz, Maurine W. 2010, Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches, Functional Ecology, vol. 24, no. 4, pp. 796-804, doi: 10.1111/j.1365-2435.2010.01689.x.

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Title Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches
Author(s) Klaassen, MarcelORCID iD for Klaassen, Marcel orcid.org/0000-0003-3907-9599
Piersma, Theunis
Korthals, Harry
Dekinga, Anne
Dietz, Maurine W.
Journal name Functional Ecology
Volume number 24
Issue number 4
Start page 796
End page 804
Total pages 9
Publisher Wiley-Blackwell Publishing
Place of publication London, England
Publication date 2010-08
ISSN 0269-8463
1365-2435
Keyword(s) isotopic clock
mathematical model
sensitivity analysis
stable carbon isotope
timing of events
Summary 1. Understanding ecological phenomena often requires an accurate assessment of the timing of events. To estimate the time since a diet shift in animals without knowledge on the isotope ratios of either the old or the new diet, isotope ratio measurements in two different tissues (e.g. blood plasma and blood cells) at a single point in time can be used. For this ‘isotopic-clock’ principle, we present here a mathematical model that yields an analytical and easily calculated outcome.

2. Compared with a previously published model, our model assumes the isotopic difference between the old and new diets to be constant if multiple measurements are taken on the same subject at different points in time. Furthermore, to estimate the time since diet switch, no knowledge of the isotopic signature of tissues under the old diet, but only under the new diet is required.

3. The two models are compared using three calibration data sets including a novel one based on a diet shift experiment in a shorebird (red knot Calidris canutus); sensitivity analyses were conducted. The two models behaved differently and each may prove rather unsatisfactory depending on the system under investigation. A single-tissue model, requiring knowledge of both the old and new diets, generally behaved quite reliably.

4. As blood (cells) and plasma are particularly useful tissues for isotopic-clock research, we trawled the literature on turnover rates in whole blood, cells and plasma. Unfortunately, turnover rate predictions using allometric relations are too unreliable to be used directly in isotopic-clock calculations.

5. We advocate that before applying the isotopic-clock methodology, the propagation of error in the ‘time-since-diet-shift’ estimation is carefully assessed for the system under scrutiny using a sensitivity analysis as proposed here.
Language eng
DOI 10.1111/j.1365-2435.2010.01689.x
Field of Research 060299 Ecology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2010, The Authors
Persistent URL http://hdl.handle.net/10536/DRO/DU:30035078

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