Bioanalytical experiments for the undergraduate laboratory : monitoring glucose in sports drinks

Gooding, J. Justin, Yang, Wenrong and Situmorang, Manihar 2001, Bioanalytical experiments for the undergraduate laboratory : monitoring glucose in sports drinks, Journal of chemical education, vol. 78, no. 6, pp. 788-790, doi: 10.1021/ed078p788.

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Title Bioanalytical experiments for the undergraduate laboratory : monitoring glucose in sports drinks
Author(s) Gooding, J. Justin
Yang, WenrongORCID iD for Yang, Wenrong
Situmorang, Manihar
Journal name Journal of chemical education
Volume number 78
Issue number 6
Start page 788
End page 790
Publisher American Chemical Society
Place of publication Bellmawr, N.J.
Publication date 2001-06
ISSN 0021-9584
Keyword(s) Electrochemistry
Summary This paper describes two complementary bioanalytical experiments for analyzing the concentration of glucose in sports drinks. The first experiment is a spectrophotometric enzyme assay employing the enzymes glucose oxidase (GOx) and horseradish peroxidase (HRP). The glucose is oxidized by the GOx, producing hydrogen peroxide, which is the substrate for HRP. In the reduction of the H2O2 a chromogen is oxidized, causing a color change. In the partner experiment, the GOx is immobilized on a platinum electrode using a dialysis membrane. The hydrogen peroxide produced in the enzyme reaction is monitored amperometrically by oxidizing the hydrogen peroxide produced. The simple method of preparing the enzyme electrode is useful in demonstrating the important parameters in defining the response of enzyme electrodes. The same sports drinks are analyzed in both experiments. The two experiments together illustrate the advantage of bioanalysis in analyzing complex samples with minimal sample preparation.
Language eng
DOI 10.1021/ed078p788
Field of Research 100703 Nanobiotechnology
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2001, American Chemical Society
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