Structure and properties of biomedical films prepared from aqueous and acidic silk fibroin solutions

Rajkhowa, Rangam, Levin, Brett, Redmond, Sharon L., Li, Lu Hua, Wang, Lijing, Kanwar, Jagat R., Atlas, Marcus D. and Wang, Xungai 2011, Structure and properties of biomedical films prepared from aqueous and acidic silk fibroin solutions, Journal of biomedical materials research : Part A, vol. 97A, no. 1, pp. 37-45, doi: 10.1002/jbm.a.33021.

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Title Structure and properties of biomedical films prepared from aqueous and acidic silk fibroin solutions
Author(s) Rajkhowa, RangamORCID iD for Rajkhowa, Rangam
Levin, Brett
Redmond, Sharon L.
Li, Lu HuaORCID iD for Li, Lu Hua
Wang, Lijing
Kanwar, Jagat R.ORCID iD for Kanwar, Jagat R.
Atlas, Marcus D.
Wang, XungaiORCID iD for Wang, Xungai
Journal name Journal of biomedical materials research : Part A
Volume number 97A
Issue number 1
Start page 37
End page 45
Total pages 9
Publisher John Wiley & Sons
Place of publication Hoboken, N.J.
Publication date 2011-04
ISSN 1549-3296
Keyword(s) silk fibroin film
tensile properties
Summary Silk fibroin films are promising materials for a range of biomedical applications. To understand the effects of casting solvents on film properties, we used water (W), formic acid (FA), and trifluoroacetic acid (TFA) as solvents. We characterized molecular weight, secondary structure, mechanical properties, and degradation behavior of cast films. Significant degradation of fibroin was observed for TFA-based film compared to W and TA-based films when analyzed by SDS-PAGE. Fibroin degradation resulted in a significant reduction in tensile strength and modulus of TFA-based films. Compared to water, TFA-based films demonstrated lower water solubility (19.6% vs. 62.5% in 12 h) despite having only a marginal increase in their ß-sheet content (26.9% vs. 23.7%). On the other hand, FA-based films with 34.3% ß-sheet were virtually water insoluble. Following solubility treatment, ß-sheet content in FA-based films increased to 50.9%. On exposure to protease XIV, water-annealed FA-based films lost 74% mass in 22 days compared to only 30% mass loss by ethanol annealed FA films. This study demonstrated that a small variation in the ß-sheet percentage and random coil conformations resulted in a significant change in the rates of enzymatic degradation without alteration to their tensile properties. The film surface roughness changed with the extent of enzymatic hydrolysis.
Language eng
DOI 10.1002/jbm.a.33021
Field of Research 090301 Biomaterials
091299 Materials Engineering not elsewhere classified
091012 Textile Technology
Socio Economic Objective 860403 Natural Fibres, Yarns and Fabrics
HERDC Research category C1 Refereed article in a scholarly journal
HERDC collection year 2011
Copyright notice ©2011, Wiley Periodicals, Inc.
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Document type: Journal Article
Collections: Centre for Material and Fibre Innovation
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