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Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system

Torriero, Angel A. J., Salinas, Eloy, Battaglini, Fernando and Raba, Julio 2003, Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system, Analytica chimica acta, vol. 498, no. 1-2, pp. 155-163, doi: 10.1016/S0003-2670(03)00897-3.

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Title Milk lactate determination with a rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system
Author(s) Torriero, Angel A. J.ORCID iD for Torriero, Angel A. J. orcid.org/0000-0001-8616-365X
Salinas, Eloy
Battaglini, Fernando
Raba, Julio
Journal name Analytica chimica acta
Volume number 498
Issue number 1-2
Start page 155
End page 163
Total pages 9
Publisher Elsevier BV
Place of publication Amsterdam, The Netherlands
Publication date 2003-11-28
ISSN 0003-2670
1873-4324
0924-2031
Keyword(s) milk
lactate
lactate oxidase
horseradish peroxidase
osmium
bioreactor
flow system
iosensors
Summary The high sensitivity that can be attained using a bienzymatic system and mediated by the redox polymer [Os(bpy)2ClPyCH2NHpoly(allylamine)] (Os-PAA), has been verified by on-line interfacing of a rotating bioreactor and continuous-flow/stopped-flow/continuous-flow processing. When the hydrogen peroxide formed by LOx layer reaches the inner layer, the electronic flow between the immobilized peroxidase and the electrode surface produces a current, proportional to lactate concentration. The determination of lactate was possible with a limit of detection of 5 nmol l−1 in the processing of as many as 30 samples per hour. This arrangement allows working in undiluted milk samples with a good stability and reproducibility. Horseradish peroxidase [EC 1.11.1.7] and Os-PAA were covalently immobilized on the glassy carbon electrode surface (upper cell body), lactate oxidase [EC 1.1.3.x] was immobilized on a disk that can be rotated.
Language eng
DOI 10.1016/S0003-2670(03)00897-3
Field of Research 030107 Sensor Technology (Chemical aspects)
030102 Electroanalytical Chemistry
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2003, Elsevier B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30038940

Document type: Journal Article
Collection: Centre for Material and Fibre Innovation
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