'Forbidden' disulfides : their role as redox switches
Wouters, M. A., George, R. A. and Haworth, N. L. 2007, 'Forbidden' disulfides : their role as redox switches, Current protein and peptide science, vol. 8, no. 5, pp. 484-495.
Attached Files
Name
Description
MIMEType
Size
Downloads
Title
'Forbidden' disulfides : their role as redox switches
Seminal studies by Richardson and Thornton defined the constraints imposed by protein structure on disulfide formation and flagged forbidden regions of primary or secondary structure seemingly incapable of forming disulfide bonds between resident cysteine pairs. With respect to secondary structure, disulfide bonds were not found between cysteine pairs: A. on adjacent beta-stands; B. in a single helix or strand; C. on non-adjacent strands of the same beta-sheet. In primary structure, disulfide bonds were not found between cysteine pairs: D. adjacent in the sequence. In the intervening years it has become apparent that all these forbidden regions are indeed occupied by disulfide-bonded cysteines, albeit rather strained ones. It has been observed that sources of strain in a protein structure, such as residues in forbidden regions of the Ramachandran plot and cis-peptide bonds, are found in functionally important regions of the protein and warrant further investigation. Like the Ramachandran plot, the earlier studies by Richardson and Thornton have identified a fundamental truth in protein stereochemistry: "forbidden" disulfides adopt strained conformations, but there is likely a functional reason for this. Emerging evidence supports a role for forbidden disulfides in redox-regulation of proteins.
Language
eng
Field of Research
060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective
970105 Expanding Knowledge in the Environmental Sciences
Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.