Peak modeling approach to accurate assignment of first-dimension retention times in comprehensive two-dimensional chromatography

Adcock, Jacqui L., Adams, Mike, Mitrevski, Blagoj S. and Marriott, Philip J. 2009, Peak modeling approach to accurate assignment of first-dimension retention times in comprehensive two-dimensional chromatography, Analytical chemistry, vol. 81, no. 16, pp. 6797-6804.

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Title Peak modeling approach to accurate assignment of first-dimension retention times in comprehensive two-dimensional chromatography
Author(s) Adcock, Jacqui L.
Adams, Mike
Mitrevski, Blagoj S.
Marriott, Philip J.
Journal name Analytical chemistry
Volume number 81
Issue number 16
Start page 6797
End page 6804
Total pages 8
Publisher American Chemical Society
Place of publication Washington, D.C.
Publication date 2009-07-23
ISSN 0003-2700
1520-6882
Summary Modeling of first-dimension retention of peaks based on modulation phase and period allows reliable prediction of the modulated peak distributions generated in the comprehensive two-dimensional chromatography experiment. By application of the inverse process, it is also possible to use the profile of the modulated peaks (their heights or areas) to predict the shape and parameters of the original input chromatographic band (retention time, standard deviation, area) for the primary column dimension. This allows an accurate derivation of the firstdimension retention time (RSD 0.02%) which is equal to that for the non-modulated experiment, rather than relying upon the retention time of the major modulated peak generated by the modulation process (RSD 0.16%). The latter metric can produce a retention time that differs by at least the modulation period employed in the experiment, which displays a discontinuity in the retention time vs modulation phase plot at the point of the 180° out-ofphase modulation. In contrast, the new procedure proposed here gives a result that is essentially independent of modulation phase and period. This permits an accurate value to be assigned to the first-dimension retention. The proposed metric accounts for the time on the seconddimension, the phase of the distribution, and the holdup time that the sampled solute is retained in the modulating interface. The approach may also be based on the largest three modulated peaks, rather than all modulated peaks. This simplifies the task of assigning the retention time with little loss of precision in band standard deviation or retention time, provided that these peaks are not all overloaded in the first or second dimension.
Language eng
Field of Research 059999 Environmental Sciences not elsewhere classified
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2009, American Chemical Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30039125

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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