Independent functions of yeast Pcf11p in pre-mRNA 3´ end processing and in transcription termination

Sadowski, Martin, Dichtl, Bernhard, Hubner, Wolfgang and Keller, Walter 2003, Independent functions of yeast Pcf11p in pre-mRNA 3´ end processing and in transcription termination, EMBO journal, vol. 22, no. 9, pp. 2167-2177, doi: 10.1093/emboj/cdg200.

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Title Independent functions of yeast Pcf11p in pre-mRNA 3´ end processing and in transcription termination
Author(s) Sadowski, Martin
Dichtl, BernhardORCID iD for Dichtl, Bernhard
Hubner, Wolfgang
Keller, Walter
Journal name EMBO journal
Volume number 22
Issue number 9
Start page 2167
End page 2177
Total pages 11
Publisher Nature Publishing Group
Place of publication London, England
Publication date 2003-05-01
ISSN 0261-4189
Keyword(s) pre-mRNA 3' end processing
RNA polymerase II C-terminal domain
transcription termination
Summary Pcf11p, an essential subunit of the yeast cleavage factor IA, is required for pre-mRNA 3' end processing, binds to the C-terminal domain (CTD) of the largest subunit of RNA polymerase II (RNAP II) and is involved in transcription termination. We show that the conserved CTD interaction domain (CID) of Pcf11p is essential for cell viability. Interestingly, the CTD binding and 3' end processing activities of Pcf11p can be functionally uncoupled from each other and provided by distinct Pcf11p fragments in trans. Impaired CTD binding did not affect the 3' end processing activity of Pcf11p and a deficiency of Pcf11p in 3' end processing did not prevent CTD binding. Transcriptional run-on analysis with the CYC1 gene revealed that loss of cleavage activity did not correlate with a defect in transcription termination, whereas loss of CTD binding did. We conclude that Pcf11p is a bifunctional protein and that transcript cleavage is not an obligatory step prior to RNAP II termination.
Language eng
DOI 10.1093/emboj/cdg200
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2003, Nature Publishing Group
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Document type: Journal Article
Collection: School of Life and Environmental Sciences
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