Properties of an in vitro selected Pb2+ cleavage motif

Pan, Tao, Dichtl, Bernhard and Uhlenbeck, Olke C. 1994, Properties of an in vitro selected Pb2+ cleavage motif, Biochemistry, vol. 33, no. 32, pp. 9561-9565.

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Title Properties of an in vitro selected Pb2+ cleavage motif
Author(s) Pan, Tao
Dichtl, Bernhard
Uhlenbeck, Olke C.
Journal name Biochemistry
Volume number 33
Issue number 32
Start page 9561
End page 9565
Total pages 5
Publisher American Chemical Society
Place of publication Washington, D. C.
Publication date 1994-08
ISSN 0006-2960
1520-4995
Keyword(s) chemical reaction
drug effect
ph
protein folding
rna cleavage
rna structure
site directed mutagenesis
Summary The addition of Pb 2+ to a small RNA molecule consisting of an asymmetric internal loop of six nucleotides results in site-specific cleavage followed by hydrolysis of the 2′,3′-cyclic phosphate intermediate [Pan, T., & Uhlenbeck, O. C. (1992) Nature 358, 560-563]. Here we show that the reaction is highly specific for Pb 2+ and the cleavage rate increases exponentially with pH from 5.5 to 7.0, both in the presence and in the absence of Mg 2+. This suggests that the reaction mechanism involves Pb 2+ hydroxide acting as a base. Several sequence variants of the RNA are found to be equally active in both steps of the reaction, suggesting that they fold into a similar structure.
Language eng
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©1994, American Chemical Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30039283

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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