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Functional analysis of yeast snoRNA and snRNA 3' end formation mediated by uncoupling of cleavage and polyadenylation

Morlando, Mariangela, Greco, Paolo, Dichtl, Bernhard, Fatica, Alessandro, Keller, Walter and Bozzoni, Irene 2002, Functional analysis of yeast snoRNA and snRNA 3' end formation mediated by uncoupling of cleavage and polyadenylation, Molecular and cellular biology, vol. 22, no. 5, pp. 1379-1389, doi: 10.1128/MCB.22.5.1379-1389.2002.

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Title Functional analysis of yeast snoRNA and snRNA 3' end formation mediated by uncoupling of cleavage and polyadenylation
Author(s) Morlando, Mariangela
Greco, Paolo
Dichtl, BernhardORCID iD for Dichtl, Bernhard orcid.org/0000-0001-5514-4982
Fatica, Alessandro
Keller, Walter
Bozzoni, Irene
Journal name Molecular and cellular biology
Volume number 22
Issue number 5
Start page 1379
End page 1389
Total pages 11
Publisher American Society for Microbiology
Place of publication Washington, D. C.
Publication date 2002-03
ISSN 0270-7306
1098-5549
Keyword(s) base sequence
fungal chromosomes
fungal proteins
fungal genes
molecular sequence data
mRNA cleavage and polyadenylation factors
mutation
nuclear proteins
polyadenylation
RNA 3' end processing
RNA precursors
saccharomyces cerevisiae
Summary Many nuclear and nucleolar small RNAs are accumulated as nonpolyadenylated species and require 3′-end processing for maturation. Here, we show that several genes coding for box C/D and H/ACA snoRNAs and for the U5 and U2 snRNAs contain sequences in their 3′ portions which direct cleavage of primary transcripts without being polyadenylated. Genetic analysis of yeasts with mutations in different components of the pre-mRNA cleavage and polyadenylation machinery suggests that this mechanism of 3"-end formation requires cleavage factor IA (CF IA) but not cleavage and polyadenylation factor activity. However, in vitro results indicate that other factors participate in the reaction besides CF IA. Sequence analysis of snoRNA genes indicated that they contain conserved motifs in their 3" noncoding regions, and mutational studies demonstrated their essential role in 3"-end formation. We propose a model in which CF IA functions in cleavage and polyadenylation of pre-mRNAs and, in combination with a different set of factors, in 3"-end formation of nonpolyadenylated polymerase II transcripts.
Language eng
DOI 10.1128/MCB.22.5.1379-1389.2002
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2002, American Society for Microbiology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30039285

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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