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Coupling between snoRNP assembly and 3' processing controls box C/D snoRNA biosynthesis in yeast

Morlando, Mariangela, Ballarino, Monica, Greco, Paolo, Caffarelli, Elisa, Dichtl, Bernhard and Bozzoni, Irene 2004, Coupling between snoRNP assembly and 3' processing controls box C/D snoRNA biosynthesis in yeast, EMBO journal, vol. 23, no. 12, pp. 2392-2401, doi: 10.1038/sj.emboj.7600254.

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Title Coupling between snoRNP assembly and 3' processing controls box C/D snoRNA biosynthesis in yeast
Author(s) Morlando, Mariangela
Ballarino, Monica
Greco, Paolo
Caffarelli, Elisa
Dichtl, BernhardORCID iD for Dichtl, Bernhard orcid.org/0000-0001-5514-4982
Bozzoni, Irene
Journal name EMBO journal
Volume number 23
Issue number 12
Start page 2392
End page 2401
Total pages 10
Publisher Nature Publishing Group
Place of publication London, England
Publication date 2004-06-16
ISSN 0261-4189
1460-2075
Keyword(s) 3'-end formation
Nop1p
Saccharomyces cerevisiae
snoRNA
snoRNP assembly
Summary RNA polymerase II transcribes genes encoding proteins and a large number of small stable RNAs. While pre-mRNA 3'-end formation requires a machinery ensuring tight coupling between cleavage and polyadenylation, small RNAs utilize polyadenylation-independent pathways. In yeast, specific factors required for snRNA and snoRNA 3'-end formation were characterized as components of the APT complex that is associated with the core complex of the cleavage/polyadenylation machinery (core-CPF). Other essential factors were identified as independent components: Nrd1p, Nab3p and Sen1p. Here we report that mutations in the conserved box D of snoRNAs and in the snoRNP-specific factor Nop1p interfere with transcription and 3'-end formation of box C/D snoRNAs. We demonstrate that Nop1p is associated with box C/D snoRNA genes and that it interacts with APT components. These data suggest a mechanism of quality control in which efficient transcription and 3'-end formation occur only when nascent snoRNAs are successfully assembled into functional particles.
Language eng
DOI 10.1038/sj.emboj.7600254
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2004, Nature Publishing Group
Persistent URL http://hdl.handle.net/10536/DRO/DU:30039286

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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