Replacement of RNA hairpins by in vitro selected tetranucleotides

Dichtl, Bernhard, Pan, Tao, di Renzo, Anthony B. and Uhlenbeck, Olke C. 1993, Replacement of RNA hairpins by in vitro selected tetranucleotides, Nucleic acids research, vol. 21, no. 3, pp. 531-535.

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Title Replacement of RNA hairpins by in vitro selected tetranucleotides
Author(s) Dichtl, Bernhard
Pan, Tao
di Renzo, Anthony B.
Uhlenbeck, Olke C.
Journal name Nucleic acids research
Volume number 21
Issue number 3
Start page 531
End page 535
Total pages 5
Publisher Oxford University Press
Place of publication Oxford, England
Publication date 1993
ISSN 0305-1048
1362-4962
Keyword(s) lead
nucleotide
rna derivative
transfer rna
Summary An in vitro selection method based on the autolytic cleavage of yeast tRNAPhe by Pb2+ was applied to obtain tRNA derivatives with the anticodon hairpin replaced by four single-stranded nucleotides. Based on the rates of the site-specific cleavage by Pb2+ and the presence of a specific UV-induced crosslink, certain tetranucieotide sequences allow proper folding of the rest of the tRNA molecule, wheras others do not. One such successful tetramer sequence was also used to replace the acceptor stem of yeast tRNAPhe and the anticodon hairpin of E.coli tRNAPhe without disrupting folding. These experiments suggest that certain tetramers may be able to replace structurally non essential hairpins in any RNA.
Language eng
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©1993, Oxford University Press
Persistent URL http://hdl.handle.net/10536/DRO/DU:30039329

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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