The solubility of Hen lysozyme in ethylammonium nitrate / H20 mixtures : a novel approach to protein crystallization

Byrne, Nolene and Angell, C. Austen 2010, The solubility of Hen lysozyme in ethylammonium nitrate / H20 mixtures : a novel approach to protein crystallization, Molecules journal, vol. 15, no. 2, pp. 793-803.

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Title The solubility of Hen lysozyme in ethylammonium nitrate / H20 mixtures : a novel approach to protein crystallization
Author(s) Byrne, Nolene
Angell, C. Austen
Journal name Molecules journal
Volume number 15
Issue number 2
Start page 793
End page 803
Total pages 11
Publisher M D P I AG
Place of publication Basel, Switzerland
Publication date 2010-02-04
ISSN 1420-3049
1431-5165
Summary We report on the solubility of hen lysozyme (HEWL) in aqueous ethylammonium nitrate (EAN) as a function of water content. We find the solubility behavior to be complex, exhibiting both a maximum (400 mg/mL) at very high EAN content) and a minimum at intermediate EAN content. We exploit this solubility profile in a novel approach to generating crystals of hydrophilic proteins, based on rehydration of a high concentration protein solution. We describe the production of crystals of X-ray diffraction quality. Two related ionic liquid solvent systems, with the same solubility profiles but different effective pH characteristics, are identified for future evaluation.
Language eng
Field of Research 039999 Chemical Sciences not elsewhere classified
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2010, M D P I AG
Persistent URL http://hdl.handle.net/10536/DRO/DU:30039447

Document type: Journal Article
Collection: Centre for Material and Fibre Innovation
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