Identification and possible disease mechanisms of an under-recognized fungus, Aureobasidium pullulans Taylor, Philip E., Esch, Robert, Flagan, Richard C., House, James, Tran, Lisa and Glovsky, M. Michael 2006, Identification and possible disease mechanisms of an under-recognized fungus, Aureobasidium pullulans, International archives of allergy and immunology, vol. 139, no. 1, pp. 45-52. Attached Files Name Description MIMEType Size Downloads Title Identification and possible disease mechanisms of an under-recognized fungus, Aureobasidium pullulans Formatted title Identification and possible disease mechanisms of an under-recognized fungus, Aureobasidium pullulans Author(s) Taylor, Philip E. orcid.org/0000-0002-4204-1893 Esch, Robert Flagan, Richard C. House, James Tran, Lisa Glovsky, M. Michael Journal name International archives of allergy and immunology Volume number 139 Issue number 1 Start page 45 End page 52 Publisher S. Karger AG Place of publication Basel, Switzerland Publication date 2006 ISSN 1018-2438 1423-0097 Keyword(s) aureobasidium pullulans yeast-like fungi respirable-sized conidia Summary Background: Investigations into the occurrence and health effects of yeast-like fungi in the outdoor air in the US have been limited. We sought to identify a respirable-sized fungus common in the Pasadena air, locate a major source for the emissions and investigate its relevance to allergic disease. Methods: Yeast-like fungi sampled from the environment were isolated, microscopically examined and sequenced. Pasadena allergy patients were skin tested with commercially available fungal extracts. Patient serum was immunoanalyzed for specific IgE reactivity. Nearby vegetation was analyzed in a controlled emission chamber to find a major source for the aerosols. Results: Hyaline unicellular conidia comprised up to 90% (41,250 m<sup>-3</sup> of air) of total fungal counts and generally peaked at night and during periods of rainfall and ensuing winds throughout the fall and winter. Flowers were determined to be a major source of the emissions. The cellular and colonial morphology of isolates were consistent with Aureobasidium species. The sequence of the D1/D2 region of the 26S ribosomal subunit of isolates from flowers showed identity to two strains of Aureohasidium pullulans (black yeast). Seventeen percent (16/94) of atopic individuals had positive skin testing with A. pullulans extract. Patient sera gE identified several high molecular weight allergens in Aureobasidium extracts. Conclusions: Respirable-sized conidia of A. pullulans are emitted from flowers and form high concentrations in the air. They are associated with immediate reactivity on skin tests, bind to patient sera IgE, and might be relevant in allergic upper and lower airway diseases. Language eng Field of Research 059999 Environmental Sciences not elsewhere classified Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©2006 S. Karger AG, Basel Persistent URL http://hdl.handle.net/10536/DRO/DU:30039857 Document type: Journal Article Collection: School of Life and Environmental Sciences Related Links Link Description Connect to published version (restricted access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Wed, 02 Nov 2011, 08:40:55 EST Thu, 03 Nov 2011, 09:04:04 EST Thu, 03 Nov 2011, 09:08:43 EST Thu, 03 Nov 2011, 09:15:04 EST Thu, 03 Nov 2011, 09:16:15 EST Thu, 02 Feb 2012, 14:32:48 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 11 times in Scopus Search Google Scholar Access Statistics: 223 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Wed, 02 Nov 2011, 08:40:55 EST

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