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The male germ unit of Rhododendron : quantitative cytology, three-dimensional reconstruction, isolation and detection using fluorescent probes

Taylor, P., Kenrick, J., Li, Y., Kaul, V., Gunning, B. E. S. and Knox, R. B. 1989, The male germ unit of Rhododendron : quantitative cytology, three-dimensional reconstruction, isolation and detection using fluorescent probes, Sexual plant reproduction, vol. 2, no. 4, pp. 254-264, doi: 10.1007/BF00195585.

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Title The male germ unit of Rhododendron : quantitative cytology, three-dimensional reconstruction, isolation and detection using fluorescent probes
Author(s) Taylor, P.ORCID iD for Taylor, P. orcid.org/0000-0002-4204-1893
Kenrick, J.
Li, Y.
Kaul, V.
Gunning, B. E. S.
Knox, R. B.
Journal name Sexual plant reproduction
Volume number 2
Issue number 4
Start page 254
End page 264
Total pages 11
Publisher Springer
Place of publication Heidelberg , Germany
Publication date 1989-11
ISSN 0934-0882
1432-2145
Keyword(s) generative cell
sperm cell
quantitative cytology
mitochondria
plastids
immunofluorescence
actin
tubulin
video-image processing.
Summary The sperm cells of Rhododendron laetum and R. macgregoriae differentiate within the pollen tube about 24 h after germination in vitro. Threedimensional reconstruction shows that the sperm cells are paired together, and both have extensions that link with the tube nucleus, forming a male germ unit. Quantitative analysis shows that the sperm cells in each pair differ significantly in surface area, but not in cell volume nor in numbers of mitochondria or plastids. When isolated from pollen tubes by osmotic shock, the sperm cells became ellipsoidal and surrounded by their own plasma membrane, while a proportion remained in pairs linked by the inner tube plasma membrane. Both generative and sperm cells are visualized in pollen tube preparations by immunofluorescence with anti-tubulin and anti-actin monoclonal antibodies (MAbs) combined with H33258 fluorescence of the nuclei. Video-image processing shows the presence of an axial microtubule cage in the generative cells, and some microtubules are present in the cytoplasmic extensions that clasp the tube nucleus. Following sperm cell division, the extensive phragmoplast between the sperm nuclei is partitioned by the plasma membranes.
Language eng
DOI 10.1007/BF00195585
Field of Research 059999 Environmental Sciences not elsewhere classified
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30039862

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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