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Sperm cells of the pollen tubes of Brassica : ultrastructure and isolation

Taylor, P. E., Kenrick, J., Blomstedt, C. K. and Knox, R. B. 1991, Sperm cells of the pollen tubes of Brassica : ultrastructure and isolation, Sexual plant reproduction, vol. 4, no. 3, pp. 226-234, doi: 10.1007/BF00190009.

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Title Sperm cells of the pollen tubes of Brassica : ultrastructure and isolation
Author(s) Taylor, P. E.ORCID iD for Taylor, P. E. orcid.org/0000-0002-4204-1893
Kenrick, J.
Blomstedt, C. K.
Knox, R. B.
Journal name Sexual plant reproduction
Volume number 4
Issue number 3
Start page 226
End page 234
Total pages 9
Publisher Springer
Place of publication Heidelberg, Germany
Publication date 1991-07
ISSN 0934-0882
1432-2145
Keyword(s) male germ unit
sperm cells
isolation
pollen tubes
brassica napus
pollen-tube inner plasma membrane
ultrastructure
Summary Sperm cells of pollen tubes grown both in vivo and in vitro form a male germ unit. Extensions from both sperm cells of each pollen tube are closely associated with the tube nucleus. A high yield (2.7 × 104. 20 mg−1 pollen grains germinated) of intact sperm cells was obtained following release by osmotic shock from pollen tubes grown in vitro. Structural integrity of isolated sperm was maintained by isolation at low temperature in an osmotically balanced medium. At 4° C many isolated sperm pairs were still enclosed within the pollentube inner plasma membrane. Sperm cells not enclosed within this membrane no longer remained connected as a pair. During isolation vesicles formed on the sperm cell surface from disruption of the fibrillar components bridging the periplasmic space. Both in the pollen tube and after isolation the sperm nucleus is in close association with at least one region of the sperm plasma membrane. Sperm isolated at room temperature showed the presence of nucleopores, and nuclei were euchromatic, instead of heterochromatic as in intact sperm in the pollen tube.
Language eng
DOI 10.1007/BF00190009
Field of Research 059999 Environmental Sciences not elsewhere classified
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30039863

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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