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Cellular localization of water soluble, allergenic proteins in rye-grass (Lolium perenne) pollen using monoclonal and specific IgE antibodies with immunogold probes

Staff, I. A., Taylor, P. E., Smith, P., Singh, M. B. and Knox, R. B. 1990, Cellular localization of water soluble, allergenic proteins in rye-grass (Lolium perenne) pollen using monoclonal and specific IgE antibodies with immunogold probes, Journal of molecular histology, vol. 22, no. 5, pp. 276-290, doi: 10.1007/BF01387183.

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Title Cellular localization of water soluble, allergenic proteins in rye-grass (Lolium perenne) pollen using monoclonal and specific IgE antibodies with immunogold probes
Author(s) Staff, I. A.
Taylor, P. E.ORCID iD for Taylor, P. E. orcid.org/0000-0002-4204-1893
Smith, P.
Singh, M. B.
Knox, R. B.
Journal name Journal of molecular histology
Volume number 22
Issue number 5
Start page 276
End page 290
Total pages 15
Publisher Springer Netherlands
Place of publication Dordrecht , Netherlands
Publication date 1990-05
ISSN 1567-2379
1567-2387
Summary A postembedding method has been developed for localizing water soluble allergens in rye-grass pollen. This uses dry fixation in glutaraldehyde vapour, followed by 2,2-dimethoxypropane, prior to a 100% ethanol series leading into embedment in LR Gold. This has allowed the attachment of specific monoclonal antibodies to the allergen, which are themselves probed with specific immunogold labels to the antibodies. Wall and cytoplasmic sites have been identified, representing an improvement of fixation and localization of allergens over previous studies employing polyclonal, broad spectrum antibodies.

Rye-grass allergens are labelled in mature pollen grains in the exine (tectum, nexine and central chamber), and in the electron opaque areas of the cytoplasm, especially mitochondria. The allergens are absent from the intine, polysaccharide (P) particles, amyloplasts, Golgi bodies and endoplasmic reticulum. IgE antibodies derived from humans allergic to rye-grass pollen, bind to similar sites in the cytoplasm but only to the outer surface of the pollen grain wall. This method now provides a valuable tool for further developmental studies on the pollen grains, in order to establish the site/s of synthesis of the allergens.
Language eng
DOI 10.1007/BF01387183
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30039870

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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