You are not logged in.

Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen : intracellular targeting to the amyloplast

Singh, Mohan B., Hough, Terryn, Theerakulpisut, Piyada, Avjioglu, Asil, Davies, Sean, Smith, Penelope M., Taylor, Philip, Simpson, Richard J., Ward, Larry D., McClusky, James, Puy, Robert and Knox, R. Bruce 1991, Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen : intracellular targeting to the amyloplast, Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 4, pp. 1384-1388, doi: 10.1073/pnas.88.4.1384.

Attached Files
Name Description MIMEType Size Downloads

Title Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen : intracellular targeting to the amyloplast
Author(s) Singh, Mohan B.
Hough, Terryn
Theerakulpisut, Piyada
Avjioglu, Asil
Davies, Sean
Smith, Penelope M.
Taylor, PhilipORCID iD for Taylor, Philip orcid.org/0000-0002-4204-1893
Simpson, Richard J.
Ward, Larry D.
McClusky, James
Puy, Robert
Knox, R. Bruce
Journal name Proceedings of the National Academy of Sciences of the United States of America
Volume number 88
Issue number 4
Start page 1384
End page 1388
Total pages 5
Publisher National Academy of Sciences
Place of publication Washington, D.C.
Publication date 1991-02
ISSN 0027-8424
1091-6490
Keyword(s) IgE binding
molecular cloning
Lolium perenne
Lol pI allergen
immunogold probes
Summary We have identified a major allergenic protein from rye-grass pollen, tentatively designated Lol pIb of 31kDa and with pI 9.0. A cDNA clone encoding Lol pIb has been isolated, sequenced, and characterized. Lol pIb is located mainly in the starch granules. This is a distinct allergen from Lol pI, which is located in the cytosol. Lol pIb is synthesized in pollen as a pre-allergen with a transit peptide targeting the allergen to amyloplasts. Epitope mapping of the fusion protein localized the IgE binding determinant in the C-terminal domain.
Language eng
DOI 10.1073/pnas.88.4.1384
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30039874

Document type: Journal Article
Collection: School of Life and Environmental Sciences
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 153 times in TR Web of Science
Scopus Citation Count Cited 131 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 160 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Wed, 02 Nov 2011, 08:41:46 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.