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Isolation of sperms from the pollen tube of flowering plants during fertilization

Shivanna, K. R., Xu, H., Taylor, P. and Knox, R. B. 1988, Isolation of sperms from the pollen tube of flowering plants during fertilization, Plant physiology, vol. 87, no. 3, pp. 647-650, doi: 10.1104/pp.87.3.647.

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Title Isolation of sperms from the pollen tube of flowering plants during fertilization
Author(s) Shivanna, K. R.
Xu, H.
Taylor, P.ORCID iD for Taylor, P. orcid.org/0000-0002-4204-1893
Knox, R. B.
Journal name Plant physiology
Volume number 87
Issue number 3
Start page 647
End page 650
Total pages 4
Publisher American Society of Plant Biologists
Place of publication Rockville, Md.
Publication date 1988-07
ISSN 0032-0889
1532-2548
Keyword(s) pollen tubes
sperm cells
pollination
fertilization
Summary Sperm cells have been isolated from pollen tubes growing in style segments of the dicotlyledon Rhododendron macgregoriae and the monocotyledon Gladiolus gandavensis by the in vivo/in vitro method at various stages of fertilization. Pollen tubes emerged from the cut end of the style into agar medium, and more than 95% contained sperm cells. Sperm cells were released from the pollen tubes by osmotic shock or by placing styles in wall-degrading enzymes: 0.5% macerozyme and 1% cellulase. The isolated sperms were ellipsoidal protoplasts of diameter about 2 × 3 micrometers in Gladiolus and about 3 × 4 micrometers in Rhododendron. After isolation, a proportion of the sperm cells occurred in pairs linked at one end by finger-like connections. The pairs of isolated sperms were dimorphic in terms of surface area and volume. By cutting the styles at various positions and times after pollination, the potential exists to detect changes in sperm gene expression associated with fertilization.
Language eng
DOI 10.1104/pp.87.3.647
Field of Research 059999 Environmental Sciences not elsewhere classified
Socio Economic Objective 970105 Expanding Knowledge in the Environmental Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©1988, American Society of Plant Biologists
Persistent URL http://hdl.handle.net/10536/DRO/DU:30039875

Document type: Journal Article
Collections: School of Life and Environmental Sciences
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.