Immunologic significance of respirable atmospheric starch granules containing major birch allergen Bet v 1

Schappi, G. F., Taylor, P. E., Staff, I. A., Rolland, J. M. and Suphioglu, C 1999, Immunologic significance of respirable atmospheric starch granules containing major birch allergen Bet v 1, Allergy, vol. 54, no. 5, pp. 478-483, doi: 10.1034/j.1398-9995.1999.00838.x.

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Title Immunologic significance of respirable atmospheric starch granules containing major birch allergen Bet v 1
Author(s) Schappi, G. F.
Taylor, P. E.ORCID iD for Taylor, P. E.
Staff, I. A.
Rolland, J. M.
Suphioglu, C
Journal name Allergy
Volume number 54
Issue number 5
Start page 478
End page 483
Total pages 6
Publisher Wiley-Blackwell
Place of publication West Sussex, England
Publication date 1999
ISSN 0105-4538
Keyword(s) allergens
starch granules
respirable fine particles
micronic particles
birch pollen
Bet v 1
allergic asthma
Summary Background: Birch-pollen allergens are an important cause of early spring hay fever and allergic asthma. Recently, we reported a mechanism for the release of respirable allergenic particles from birch pollen containing the major allergen Bet v 1. In this study, we aimed to assess the immunologic significance of the released Bet v 1-containing starch granules in the environment.

Methods: A two-site monoclonal antibody-based assay (ELISA) was employed to quantitate Bet v 1 in high-volume air sampler filter extracts, and immunogold-labelling was used on sections of these extracts to localize Bet v 1. Immunoblot analyses were performed with pooled sera from patients sensitive to birch pollen.

Results: Atmospheric starch granules contained Bet v 1, and the concentration increased upon light rainfall. Sera from patients allergic to birch allergens recognized extracts from isolated starch granules.

Conclusions: The clinical implications of these findings are that starch granules released from birch pollen are potentially able to trigger allergic asthmatic reactions to Bet v 1, since the allergen occurs in respirable particles. Thus, clinicians can advise asthma patients to remain indoors on days of light rainfall during the birch-pollen season to avoid high levels of allergen exposure.
Language eng
DOI 10.1034/j.1398-9995.1999.00838.x
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
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Document type: Journal Article
Collection: School of Life and Environmental Sciences
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