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Survivin mutant protects differentiated dopaminergic SK-N-SH cells against oxidative stress

Baratchi, Sara, Kanwar, Rupinder K. and Kanwar, Jagat R. 2011, Survivin mutant protects differentiated dopaminergic SK-N-SH cells against oxidative stress, PLoS ONE, vol. 6, no. 1, pp. 1-8.

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Title Survivin mutant protects differentiated dopaminergic SK-N-SH cells against oxidative stress
Author(s) Baratchi, Sara
Kanwar, Rupinder K.
Kanwar, Jagat R.
Journal name PLoS ONE
Volume number 6
Issue number 1
Start page 1
End page 8
Total pages 8
Publisher Public Library of Science
Place of publication San Francisco, Ca.
Publication date 2011-01
ISSN 1932-6203
Summary Oxidative stress is due to an imbalance of antioxidant/pro-oxidant homeostasis and is associated with the progression of several neurological diseases, including Parkinson’s and Alzheimer’s disease and amyotrophic lateral sclerosis. Furthermore, oxidative stress is responsible for the neuronal loss and dysfunction associated with disease pathogenesis. Survivin is a member of the inhibitors of the apoptosis (IAP) family of proteins, but its neuroprotective effects have not been studied. Here, we demonstrate that SurR9-C84A, a survivin mutant, has neuroprotective effects against H2O2-induced neurotoxicity. Our results show that H2O2 toxicity is associated with an increase in cell death, mitochondrial membrane depolarisation, and the expression of cyclin D1 and caspases 9 and 3. In addition, pre-treatment with SurR9-C84A reduces cell death by decreasing both the level of mitochondrial depolarisation and the expression of cyclin D1 and caspases 9 and 3. We further show that SurR9-C84A increases the antioxidant activity of GSH-peroxidase and catalase, and effectively counteracts oxidant activity following exposure to H2O2. These results suggest for the first time that SurR9-C84A is a promising treatment to protect neuronal cells against H2O2-induced neurotoxicity.
Notes This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Language eng
Field of Research 110904 Neurology and Neuromuscular Diseases
Socio Economic Objective 920111 Nervous System and Disorders
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2011, Baratchi et al.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30040634

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.