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Interfacing cell-based assays in environmental scanning electron microscopy using dielectrophoresis

Khoshmanesh, Khashayar, Akagi, Jin, Nahavandi, Saeid, Kalantar-Zadeh, Kourosh, Baratchi, Sara, Williams, David E., Cooper, Jonathan M. and Wlodkowic, Donald 2011, Interfacing cell-based assays in environmental scanning electron microscopy using dielectrophoresis, Analytical chemistry, vol. 83, no. 8, pp. 3217-3221.

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Title Interfacing cell-based assays in environmental scanning electron microscopy using dielectrophoresis
Author(s) Khoshmanesh, Khashayar
Akagi, Jin
Nahavandi, SaeidORCID iD for Nahavandi, Saeid orcid.org/0000-0002-0360-5270
Kalantar-Zadeh, Kourosh
Baratchi, Sara
Williams, David E.
Cooper, Jonathan M.
Wlodkowic, Donald
Journal name Analytical chemistry
Volume number 83
Issue number 8
Start page 3217
End page 3221
Total pages 5
Publisher American Chemical Society
Place of publication Washington, D. C.
Publication date 2011
ISSN 0003-2700
1520-6882
Summary Development of the dielectrophoretic (DEP) live cell trapping technology and its interfacing with the environmental scanning electron microscopy (ESEM) is described. DEP microelectrode arrays were fabricated on glass substrate using photolithography and lift-off. Chip-based arrays were applied for ESEM analysis of DEP-trapped human leukemic cells. This work provides proof-of-concept interfacing of the DEP cell retention and trapping technology with ESEM to provide a high-resolution analysis of individual nonadherent cells.
Language eng
Field of Research 091306 Microelectromechanical Systems (MEMS)
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2011, American Chemical Society.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30040645

Document type: Journal Article
Collection: Centre for Intelligent Systems Research
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