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The pseudomonas aeruginosa secreted protein PA2934 decreases apical membrane expression of the cystic fibrosis transmembrane conductance regulator

MacEachran, Daniel P., Ye, Siying, Bomberger, Jennifer M., Hogan, Deborah A., Swiatecka-Urban, Agnieszka, Stanton, Bruce A. and O'Toole, George A. 2007, The pseudomonas aeruginosa secreted protein PA2934 decreases apical membrane expression of the cystic fibrosis transmembrane conductance regulator, Infection and immunity, vol. 75, no. 8, pp. 3902-3912, doi: 10.1128/IAI.00338-07.

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Title The pseudomonas aeruginosa secreted protein PA2934 decreases apical membrane expression of the cystic fibrosis transmembrane conductance regulator
Author(s) MacEachran, Daniel P.
Ye, Siying
Bomberger, Jennifer M.
Hogan, Deborah A.
Swiatecka-Urban, Agnieszka
Stanton, Bruce A.
O'Toole, George A.
Journal name Infection and immunity
Volume number 75
Issue number 8
Start page 3902
End page 3912
Publisher American Society for Microbiology
Place of publication Washington, D. C.
Publication date 2007-08
ISSN 0019-9567
1098-5522
Summary We previously reported that Pseudomonas aeruginosa PA14 secretes a protein that can reduce the apical membrane expression of the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Here we report that we have used a proteomic approach to identify this secreted protein as PA2394, and we have named the gene cif, for CFTR inhibitory factor. We demonstrate that Cif is a secreted protein and is found associated with outer membrane-derived vesicles. Expression of Cif in Escherichia coli and purification of the C-terminal six-His-tagged Cif protein showed that Cif is necessary and sufficient to mediate the reduction in apical membrane expression of CFTR and a concomitant reduction in CFTR-mediated Cl− ion secretion. Cif demonstrates epoxide hydrolase activity in vitro and requires a highly conserved histidine residue identified in α/β hydrolase family enzymes to catalyze this reaction. Mutating this histidine residue also abolishes the ability of Cif to reduce apical membrane CFTR expression. Finally, we demonstrate that the cif gene is expressed in the cystic fibrosis (CF) lung and that nonmucoid isolates of P. aeruginosa show greater expression of the gene than do mucoid isolates. We propose a model in which the Cif-mediated decrease in apical membrane expression of CFTR by environmental isolates of P. aeruginosa facilitates the colonization of the CF lung by this microbe.
Notes Article first published online 14th May 2007
Language eng
DOI 10.1128/IAI.00338-07
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2007, American Society for Microbiology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30040947

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.