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Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles

Bomberger, Jennifer M., MacEachran, Daniel P., Coutermarsh, Bonita A., Ye, Siying, O'Toole, George A. and Stanton, Bruce A. 2009, Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles, PLoS pathogens, vol. 5, no. 4, pp. 1-13, doi: 10.1371/journal.ppat.1000382.

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Title Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles
Author(s) Bomberger, Jennifer M.
MacEachran, Daniel P.
Coutermarsh, Bonita A.
Ye, Siying
O'Toole, George A.
Stanton, Bruce A.
Journal name PLoS pathogens
Volume number 5
Issue number 4
Start page 1
End page 13
Total pages 13
Publisher Public Library of Science
Place of publication San Francisco, Calif.
Publication date 2009-04-10
ISSN 1553-7366
1553-7374
Summary Bacteria use a variety of secreted virulence factors to manipulate host cells, thereby causing significant morbidity and mortality. We report a mechanism for the long-distance delivery of multiple bacterial virulence factors, simultaneously and directly into the host cell cytoplasm, thus obviating the need for direct interaction of the pathogen with the host cell to cause cytotoxicity. We show that outer membrane–derived vesicles (OMV) secreted by the opportunistic human pathogen Pseudomonas aeruginosa deliver multiple virulence factors, including b-lactamase, alkaline phosphatase, hemolytic phospholipase C, and Cif, directly into the host cytoplasm via fusion of OMV with lipid rafts in the host plasma membrane. These virulence factors enter the cytoplasm of the host cell via N-WASP–mediated actin trafficking, where they rapidly distribute to specific subcellular locations to affect host cell biology. We propose that secreted virulence factors are not released individually as naked proteins into the surrounding milieu where they may randomly contact the surface of the host cell, but instead bacterial derived OMV deliver multiple virulence factors simultaneously and directly into the host cell cytoplasm in a coordinated manner.
Language eng
DOI 10.1371/journal.ppat.1000382
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2009, PLoS
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30040949

Document type: Journal Article
Collections: School of Medicine
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.