Uncoupling the mechanisms that facilitate cell survival in hormone-deprived bovine mammary explants

Brennan, Amelia J., Sharp, Julie A., Lefèvre, Christophe M. and Nicholas, Kevin R. 2008, Uncoupling the mechanisms that facilitate cell survival in hormone-deprived bovine mammary explants, Journal of molecular endocrinology, vol. 41, no. 3, pp. 103-116.

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Title Uncoupling the mechanisms that facilitate cell survival in hormone-deprived bovine mammary explants
Author(s) Brennan, Amelia J.
Sharp, Julie A.ORCID iD for Sharp, Julie A. orcid.org/0000-0002-4481-5223
Lefèvre, Christophe M.
Nicholas, Kevin R.
Journal name Journal of molecular endocrinology
Volume number 41
Issue number 3
Start page 103
End page 116
Total pages 14
Publisher BioScientifica
Place of publication Bristol, England
Publication date 2008-09-01
ISSN 0952-5041
Keyword(s) mammary explants
Summary Mammary explants can be hormonally stimulated to mimic the biochemical changes that occur during lactogenesis. Previous studies using mammary explants concluded that the addition of exogenous macromolecules were required for mammary epithelial cells to remain viable in culture. The present study examines the survival of mammary explants from the dairy cow using milk protein gene expression as a functional marker of lactation and cell viability. Mammary explants cultured from late pregnant cows mimicked lactogenesis and showed significantly elevated milk protein gene expression after 3 days of culture with lactogenic hormones. The subsequent removal of exogenous hormones from the media for 10 days resulted in the down-regulation of milk protein genes. During this time, the mammary explants remained hormone responsive, the alveolar architecture was maintained and the expression of milk protein genes was re-induced after a second challenge with lactogenic hormones. We report that a population of bovine mammary epithelial cells have an intrinsic capacity to remain viable and hormone responsive for extended periods in chemically defined media without any exogenous macromolecules. In addition, we found mammary explant viability was dependent on de novo protein and RNA synthesis. Global functional microarray analysis showed that differential expression of genes involved in energy production, immune responses, oxidative stress and apoptosis signalling might contribute to cell survival. As the decline in milk production in dairy cattle after peak lactation results in considerable economic loss, the identification of novel survival genes may be used as genetic markers for breeding programmes to improve lactational persistency in dairy cows.

Language eng
Field of Research 060405 Gene Expression (incl Microarray and other genome-wide approaches)
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2008, Society for Endocrinology
Persistent URL http://hdl.handle.net/10536/DRO/DU:30040973

Document type: Journal Article
Collection: Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)
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Created: Wed, 07 Dec 2011, 12:51:39 EST by Julie Sharp

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