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Isolation of a novel Orientia species (O. chuto sp. nov.) from a patient infected in Dubai

Izzard, Leonard, Fuller, Andrew, Blacksell, Stuart D., Paris, Daniel H., Richards, Allen L., Aukkanit, Nuntipa, Nguyen, Chelsea, Jiang, Ju, Fenwick, Stan, Day, Nicholas P. J., Graves, Stephen and Stenos, John 2010, Isolation of a novel Orientia species (O. chuto sp. nov.) from a patient infected in Dubai, Journal of clinical microbiology, vol. 48, no. 12, pp. 4404-4409, doi: 10.1128/JCM.01526-10.

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Title Isolation of a novel Orientia species (O. chuto sp. nov.) from a patient infected in Dubai
Author(s) Izzard, Leonard
Fuller, Andrew
Blacksell, Stuart D.
Paris, Daniel H.
Richards, Allen L.
Aukkanit, Nuntipa
Nguyen, Chelsea
Jiang, Ju
Fenwick, Stan
Day, Nicholas P. J.
Graves, Stephen
Stenos, John
Journal name Journal of clinical microbiology
Volume number 48
Issue number 12
Start page 4404
End page 4409
Total pages 6
Publisher American Society for Microbiology
Place of publication Washington, D. C.
Publication date 2010-12
ISSN 0095-1137
1098-660X
Summary In July 2006, an Australian tourist returning from Dubai, in the United Arab Emirates (UAE), developed acute scrub typhus. Her signs and symptoms included fever, myalgia, headache, rash, and eschar. Orientia tsutsugamushi serology demonstrated a 4-fold rise in antibody titers in paired serum collections (1:512 to 1:8,192), with the sera reacting strongest against the Gilliam strain antigen. An Orientia species was isolated by the in vitro culture of the patient's acute blood taken prior to antibiotic treatment. The gene sequencing of the 16S rRNA gene (rrs), partial 56-kDa gene, and the full open reading frame 47-kDa gene was performed, and comparisons of this new Orientia sp. isolate to previously characterized strains demonstrated significant sequence diversity. The closest homology to the rrs sequence of the new Orientia sp. isolate was with three strains of O. tsutsugamushi (Ikeda, Kato, and Karp), with a nucleotide sequence similarity of 98.5%. The closest homology to the 47-kDa gene sequence was with O. tsutsugamushi strain Gilliam, with a nucleotide similarity of 82.3%, while the closest homology to the 56-kDa gene sequence was with O. tsutsugamushi strain TA686, with a nucleotide similarity of 53.1%. The molecular divergence and geographically unique origin lead us to believe that this organism should be considered a novel species. Therefore, we have proposed the name “Orientia chuto,” and the prototype strain of this species is strain Dubai, named after the location in which the patient was infected.
Notes Article first published online 6th October 2010
Language eng
DOI 10.1128/JCM.01526-10
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2010, American Society for Microbiology. All Rights Reserved.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30041595

Document type: Journal Article
Collection: School of Medicine
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