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Characterization of Type 1 ribosome inactivating proteins in edible plants

Puri, Munish, Kaur, Inderdeep and Barrow, C. J. 2011, Characterization of Type 1 ribosome inactivating proteins in edible plants, in ICAAP 2011 : 12th International Conference on amino acids, peptides and proteins, ICAAP, Beijing, China.

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Title Characterization of Type 1 ribosome inactivating proteins in edible plants
Author(s) Puri, Munish
Kaur, Inderdeep
Barrow, C. J.
Conference name International Conference on amino acids, peptides and proteins (12th : 2011 : Beijing, China)
Conference location Beijing, China
Conference dates 1-5 Aug. 2011
Title of proceedings ICAAP 2011 : 12th International Conference on amino acids, peptides and proteins
Editor(s) [Unknown]
Publication date 2011
Conference series International Conference on amino acids, peptides and proteins
Publisher ICAAP
Place of publication Beijing, China
Summary The ribosome inactivating proteins (RIPs) from plants possess RNA N-glycosidase activity that depurinates the major rRNA, thus damaging ribosome in an irreversible manner and arresting protein synthesis. RIPs occur in fungi, bacteria and plants and are abundant in angiosperms, where they appear to have defensive role. RIPs are presently classified as rRNA N-glycosidase in the enzyme nomenclature (EC 3.2.2.22) and do exhibit other enzymatic activities such as ribonuclease and deoxyribonuclease activities. RIPs are classified into two groups based on their difference in their primary structure. Type I RIPs consist of a single polypeptide chain of approximately 26–35 kDa that possess an RNA N-glycosidase activity. These proteins have attracted a great deal of attention because of their anti-viral, anti-tumor, and anti-microbial activities, which is useful in medical research and development. Here, we describe isolation of a novel protein from Momordica sp, a highclimbing vine from family Cucurbitaceae which is native to the tropical regions of Africa, Asia, Arabia and Caribbean. The purified protein has been verified by SDS-PAGE and mass spectrometry to contain only single chain Type-1 ribosome inactivating proteins (RIPs). With present experiments, we determined the presence of RIPs in edible plant materials, including some that are eaten raw by human beings. The novel protein is further characterized to validate its therapeutic potential.
Language eng
Field of Research 100302 Bioprocessing, Bioproduction and Bioproducts
Socio Economic Objective 860105 Nutraceuticals and Functional Foods
HERDC Research category E2 Full written paper - non-refereed / Abstract reviewed
Copyright notice ©2011, ICAAP
Persistent URL http://hdl.handle.net/10536/DRO/DU:30042213

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