The whole-body fatty acid balance method : examples of its potential for feed efficiency and product quality optimisation in fish and poultry
Turchini, G. M., De Smet, S. and Francis, D. S. 2011, The whole-body fatty acid balance method : examples of its potential for feed efficiency and product quality optimisation in fish and poultry, in Recent advances in animal nutrition - Australia, v.18, Animal Science, University of New England, Armidale, N. S. W., pp. 69-76.
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The whole-body fatty acid balance method : examples of its potential for feed efficiency and product quality optimisation in fish and poultry
Dietary lipids and fatty acids are not only fundamental in determining animal performance, but also determine the eating qualities of animal products. Several methods have been used to quantify fatty acid metabolism but most involve expensive in vitro approaches that are not suitable for most laboratories. Furthermore, there is considerable variation between methods with regard to enzyme activity, which makes comparison of results between studies difficult. The recently developed whole-body fatty acid balance method (WBFABM) is a simple and reliable in vivo method for assessing fatty acid metabolism, including rates of liponeogenesis and de novo fatty acid production, β-oxidation of fatty acids and bioconversion (elongation and desaturation) of fatty acids to long-chain polyunsaturated fatty acids. Initially developed for implementation with a fish model, the WBFABM has proven to be a simple and effective method that can be used in any laboratory equipped with a gas chromatography unit. Since its development, it has been used in several farmed finfish feeding trials and in broiler chicken feeding trials. The WBFABM is currently used at research institutions worldwide and its use is increasing in popularity among animal scientists. With this method, it is possible to track the fate of individual dietary fatty acids within the body. The WBFABM could contribute significantly to information generated by animal feeding trials.