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The effect of cocoa supplementation on hepatic steatosis, reactive oxygen species and LFABP in a rat model of NASH

Janevski, Mile, Antonas, Kiriakos N., Sullivan-Gunn, Melanie J., McGlynn, Maree A. and Lewandowski, Paul A. 2011, The effect of cocoa supplementation on hepatic steatosis, reactive oxygen species and LFABP in a rat model of NASH, Comparative hepatology, vol. 10, no. 10, pp. 1-13.

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Title The effect of cocoa supplementation on hepatic steatosis, reactive oxygen species and LFABP in a rat model of NASH
Author(s) Janevski, Mile
Antonas, Kiriakos N.
Sullivan-Gunn, Melanie J.
McGlynn, Maree A.
Lewandowski, Paul A.
Journal name Comparative hepatology
Volume number 10
Issue number 10
Start page 1
End page 13
Total pages 13
Publisher BioMed Central
Place of publication London, U. K.
Publication date 2011-11-14
ISSN 1476-5926
Keyword(s) non alcoholic steatohepatitis
NASH
oxidative stress
antioxidant
liver fatty acid binding protein
NADPH oxidase
cocoa
Summary Background: Non alcoholic steatohepatitis is hypothesised to develop via a mechanism involving fat accumulation and oxidative stress. The current study aimed to investigate if an increase in oxidative stress was associated with changes in the expression of liver fatty acid binding protein in a rat model of non alcoholic steatohepatitis and whether cocoa supplementation attenuated those changes.

Methods: Female Sprague Dawley rats were fed a high fat control diet, a high fat methionine choline deficient diet, or one of four 12.5% cocoa supplementation regimes in combination with the high fat methionine choline deficient diet.

Results: Liver fatty acid binding protein mRNA and protein levels were reduced in the liver of animals with fatty liver disease when compared to controls. Increased hepatic fat content was accompanied by higher levels of oxidative stress in animals with fatty liver disease when compared to controls. An inverse association was found between the levels of hepatic liver fatty acid binding protein and the level of hepatic oxidative stress in fatty liver disease. Elevated NADPH oxidase protein levels were detected in the liver of animals with increased severity in inflammation and fibrosis. Cocoa supplementation was associated with partial attenuation of these pathological changes, although the severity of liver disease induced by the methionine choline deficient diet prevented complete reversal of any disease associated changes. Red blood cell glutathione was increased by cocoa supplementation, whereas liver glutathione was reduced by cocoa compared to methionine choline deficient diet fed animals.

Conclusion: These findings suggest a potential role for liver fatty acid binding protein and NADPH oxidase in the development of non alcoholic steatohepatitis. Furthermore, cocoa supplementation may have be of therapeutic benefit in less sever forms of NASH.
Notes This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Language eng
Field of Research 111103 Nutritional Physiology
Socio Economic Objective 920411 Nutrition
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2011, Janevski et al.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30042978

Document type: Journal Article
Collections: School of Medicine
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