Effect of L-arginine infusion on glucose disposal during exercise in humans

Linden, Kelly C., Wadley, Glenn D., Garnham, Andrew P. and McConell, Glenn K. 2011, Effect of L-arginine infusion on glucose disposal during exercise in humans, Medicine and science in sports and exercise, vol. 43, no. 9, pp. 1626-1634.

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Title Effect of L-arginine infusion on glucose disposal during exercise in humans
Author(s) Linden, Kelly C.
Wadley, Glenn D.
Garnham, Andrew P.
McConell, Glenn K.
Journal name Medicine and science in sports and exercise
Volume number 43
Issue number 9
Start page 1626
End page 1634
Publisher Lippincott Williams & Wilkins
Place of publication Philadelphia, Pa.
Publication date 2011-09
ISSN 0195-9131
1530-0315
Keyword(s) nitric oxide
contraction
AKT
insulin
Summary Purpose: We have previously shown that local infusion of a nitric oxide synthase (NOS) inhibitor attenuates increases in leg glucose uptake during exercise in humans. We have also shown that infusion of the NOS substrate, L-arginine (L-Arg), increases glucose clearance, although the mechanisms involved were not determined. A potential mechanism for NO-mediated glucose disposal is via interactions with NOS and the energy sensor AMPactivated protein kinase (AMPK). The aim of this study was to determine the mechanism(s) by which L-Arg infusion increases glucose disposal during exercise in humans by examining total NOS activity and AMPK signaling.

Methods: Seven males cycled for 120 min at 64% T 1% V˙ O2peak, during which the [6,6-2H]glucose tracer was infused. During the final 60 min of exercise, either saline alone (Control, CON), or saline containing L-Arg HCl (L-Arg, 30 g at 0.5 gIminj1) was coinfused in a double-blind, randomized, counterbalanced order.

Results: L-Arg increased the glucose rate of disappearance and glucose clearance rate during exercise; however, this was accompanied by a 150% increase in plasma insulin concentration from 65 to 75 min (P G 0.05) that remained significantly elevated until 90 min of exercise. Skeletal muscle AMPK signaling, nNOSK phosphorylation by AMPK, and total NOS activity increased to a similar extent in the two trials.

Conclusions: The increase in glucose disposal after L-Arg infusion during exercise is likely due to the significantly higher plasma insulin concentration.
Language eng
Field of Research 110602 Exercise Physiology
Socio Economic Objective 920104 Diabetes
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2011, American College of Sports Medicine
Persistent URL http://hdl.handle.net/10536/DRO/DU:30043843

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