Effect of L-arginine infusion on glucose disposal during exercise in humans

Linden, Kelly C., Wadley, Glenn D., Garnham, Andrew P. and McConell, Glenn K. 2011, Effect of L-arginine infusion on glucose disposal during exercise in humans, Medicine and science in sports and exercise, vol. 43, no. 9, pp. 1626-1634, doi: 10.1249/MSS.0b013e318212a317.

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Title Effect of L-arginine infusion on glucose disposal during exercise in humans
Author(s) Linden, Kelly C.
Wadley, Glenn D.ORCID iD for Wadley, Glenn D. orcid.org/0000-0002-6617-4359
Garnham, Andrew P.
McConell, Glenn K.
Journal name Medicine and science in sports and exercise
Volume number 43
Issue number 9
Start page 1626
End page 1634
Total pages 9
Publisher Lippincott Williams & Wilkins
Place of publication Philadelphia, Pa.
Publication date 2011-09
ISSN 0195-9131
Keyword(s) nitric oxide
Summary Purpose: We have previously shown that local infusion of a nitric oxide synthase (NOS) inhibitor attenuates increases in leg glucose uptake during exercise in humans. We have also shown that infusion of the NOS substrate, L-arginine (L-Arg), increases glucose clearance, although the mechanisms involved were not determined. A potential mechanism for NO-mediated glucose disposal is via interactions with NOS and the energy sensor AMPactivated protein kinase (AMPK). The aim of this study was to determine the mechanism(s) by which L-Arg infusion increases glucose disposal during exercise in humans by examining total NOS activity and AMPK signaling.

Methods: Seven males cycled for 120 min at 64% T 1% V˙ O2peak, during which the [6,6-2H]glucose tracer was infused. During the final 60 min of exercise, either saline alone (Control, CON), or saline containing L-Arg HCl (L-Arg, 30 g at 0.5 gIminj1) was coinfused in a double-blind, randomized, counterbalanced order.

Results: L-Arg increased the glucose rate of disappearance and glucose clearance rate during exercise; however, this was accompanied by a 150% increase in plasma insulin concentration from 65 to 75 min (P G 0.05) that remained significantly elevated until 90 min of exercise. Skeletal muscle AMPK signaling, nNOSK phosphorylation by AMPK, and total NOS activity increased to a similar extent in the two trials.

Conclusions: The increase in glucose disposal after L-Arg infusion during exercise is likely due to the significantly higher plasma insulin concentration.
Language eng
DOI 10.1249/MSS.0b013e318212a317
Field of Research 110602 Exercise Physiology
Socio Economic Objective 920104 Diabetes
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2011, American College of Sports Medicine
Persistent URL http://hdl.handle.net/10536/DRO/DU:30043843

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