Tammar wallaby mammary cathelicidins are differentially expressed during lactation and exhibit antimicrobial and cell proliferative activity

Wanyonyi, Stephen S., Sharp, Julie A., Khalil, Elie, Lefevre, Christophe and Nicholas, Kevin R. 2011, Tammar wallaby mammary cathelicidins are differentially expressed during lactation and exhibit antimicrobial and cell proliferative activity, Comparative biochemistry and physiology. Part A : molecular & integrative physiology, vol. 160, no. 3, pp. 431-439.

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Title Tammar wallaby mammary cathelicidins are differentially expressed during lactation and exhibit antimicrobial and cell proliferative activity
Author(s) Wanyonyi, Stephen S.
Sharp, Julie A.
Khalil, Elie
Lefevre, Christophe
Nicholas, Kevin R.
Journal name Comparative biochemistry and physiology. Part A : molecular & integrative physiology
Volume number 160
Issue number 3
Start page 431
End page 439
Total pages 9
Publisher Elsevier
Place of publication New York, N. Y.
Publication date 2011
ISSN 1095-6433
1531-4332
Keyword(s) antimicrobial
cathelicidin
cell proliferation
mammary epithelial cells
tammar wallaby
Summary Cathelicidins secreted in milk may be central to autocrine feedback in the mammary gland for optimal development in addition to conferring innate immunity to both the mammary gland and the neonate. This study exploits the unique reproductive strategy of the tammar wallaby (Macropus eugenii) model to analyse differential splicing of cathelicidin genes and to evaluate the bactericidal activity and effect of the protein on mammary epithelial cell proliferation. Two linear peptides, Con73 and Con218, derived from the heterogeneous carboxyl end of cathelicidin transcripts, MaeuCath1 and MaeuCath7 respectively, were evaluated for antimicrobial activity. Both Con73 and Con218 significantly inhibited the growth of Staphylococcus aureus, Pseudomonas aureginosa, Enterococcus faecalis and Salmonella enterica. In addition both MaeuCath1 and MaeuCath7 stimulated proliferation of primary tammar wallaby mammary epithelial cells (WallMEC). Lactation-phase specific alternate spliced transcripts were determined for MaeuCath1 showing utilisation of both antimicrobial and proliferative functions are required by the mammary gland and the suckled young. The study has shown for the first time that temporal regulation of milk cathelicidins may be crucial in antimicrobial protection of the mammary gland and suckled young and mammary cell proliferation.
Language eng
Field of Research 060408 Genomics
060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 860105 Nutraceuticals and Functional Foods
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2011, Elsevier
Persistent URL http://hdl.handle.net/10536/DRO/DU:30044133

Document type: Journal Article
Collection: Centre for Biotechnology and Interdisciplinary Sciences (BioDeakin)
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