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The development of 20 microsatellite loci for the Australian marine mollusk, Donax deltoides, through next generation DNA sequencing

Miller, Adam D., Versace, Vincent L., Matthews, Ty and Bowie, Kate C. 2012, The development of 20 microsatellite loci for the Australian marine mollusk, Donax deltoides, through next generation DNA sequencing, Conservation genetics resources, vol. 4, no. 2, pp. 257-260.

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Title The development of 20 microsatellite loci for the Australian marine mollusk, Donax deltoides, through next generation DNA sequencing
Formatted title The development of 20 microsatellite loci for the Australian marine mollusk, Donax deltoides, through next generation DNA sequencing
Author(s) Miller, Adam D.ORCID iD for Miller, Adam D. orcid.org/0000-0002-1632-7206
Versace, Vincent L.ORCID iD for Versace, Vincent L. orcid.org/0000-0002-8514-1763
Matthews, Ty
Bowie, Kate C.
Journal name Conservation genetics resources
Volume number 4
Issue number 2
Start page 257
End page 260
Total pages 4
Publisher Springer Netherlands
Place of publication Dordrecht, The Netherlands
Publication date 2012-06
ISSN 1877-7252
1877-7260
Keyword(s) microsatellite development
next generation DNA sequencing
donax deltoids
marine mollusk
Summary  Next Generation DNA sequencing was used to develop a suite of microsatellite markers for the marine mollusk, Donax deltoides. A total of 20 polymorphic loci were identified and 12 characterized using 30 individuals from a single population (Venus Bay) in south eastern Australia. We observed moderate to high genetic variation across most loci (mean number of alleles per locus = 7.3; mean heterozygosity = 0.633) with only a single locus (Ddel32) displaying significant deviation from Hardy–Weinberg equilibrium. Marker independence was confirmed with tests for linkage disequilibrium, however two loci were found to be influenced by null alleles. The 10 viable markers characterized in the present study provide a valuable resource for future population genetic assessments and fisheries management of D. deltoides in Australia.
Language eng
Field of Research 050202 Conservation and Biodiversity
060499 Genetics not elsewhere classified
070403 Fisheries Management
Socio Economic Objective 960802 Coastal and Estuarine Flora, Fauna and Biodiversity
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2011, Springer Science+Business Media B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30044139

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