Fish oil replacement in rainbow trout diets and total dietary PUFA content : II) Effects on fatty acid metabolism and in vivo fatty acid bioconversion

Thanuthong, Thanongsak, Francis, David S., Manickam, Elizabeth, Senadheera, Shyamalie D., Cameron-Smith, David and Turchini, Giovanni M. 2011, Fish oil replacement in rainbow trout diets and total dietary PUFA content : II) Effects on fatty acid metabolism and in vivo fatty acid bioconversion, Aquaculture, vol. 322-323, pp. 99-108.

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Title Fish oil replacement in rainbow trout diets and total dietary PUFA content : II) Effects on fatty acid metabolism and in vivo fatty acid bioconversion
Author(s) Thanuthong, Thanongsak
Francis, David S.
Manickam, Elizabeth
Senadheera, Shyamalie D.
Cameron-Smith, David
Turchini, Giovanni M.
Journal name Aquaculture
Volume number 322-323
Start page 99
End page 108
Total pages 10
Publisher Elsevier BV
Place of publication Amsterdam, The Netherlands
Publication date 2011-12-21
ISSN 0044-8486
1873-5622
Keyword(s) alpha-linolenic acid (18:3n-3)
desaturase
elongase
fish oil replacement
linoleic acid (18:2n-6)
Summary This study aimed to gain a better understanding of the metabolic fate of dietary fatty acids in rainbow trout, with a specific focus on the effect of varying total C18 PUFA level. Fish were fed a control fish oil based diet or one of five experimental fish oil deprived diets formulated with a constant 1/1 ratio of 18:3n-3/18:2n-6 and varying total C18 PUFA levels for a period of 7 weeks. The transcriptional changes of the Δ-6 desaturase and elongase enzymes in direct comparison to in vivo fatty acid bioconversion, estimated using the whole-body fatty acid balance method, were analysed. The main findings were that i) the efficiency of Δ-6 desaturase was negatively affected by C18 PUFA availability, but the total apparent in vivo enzyme activity was directly proportional to C18 PUFA substrate availability; ii) Δ-6 desaturase had a greater affinity towards n-3PUFA than n-6PUFA; iii) excessive C18 PUFA substrate availability could limit the availability of Δ-6 desaturase to act on C24 fatty acid; iv) the elimination of dietary n-3LC-PUFA (enzyme products) up-regulated the transcription rate of Δ-6 desaturase; but v) the total apparent in vivo enzyme activity was directly and positively affected by substrate availability, and not product presence/absence nor the extent of the enzyme transcription rate.
Language eng
Field of Research 070401 Aquaculture
Socio Economic Objective 830102 Aquaculture Fin Fish (excl. Tuna)
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2011, Elsevier B.V.
Persistent URL http://hdl.handle.net/10536/DRO/DU:30044373

Document type: Journal Article
Collection: School of Life and Environmental Sciences
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