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Effect of naloxone-precipitated morphine withdrawal on c-fos expression in rat corticotropin-releasing hormone neurons in the paraventricular hypothalamus and extended amygdala

Hamlin, A. S., Buller, K. M., Day, T. A. and Osborne, P. B. 2004, Effect of naloxone-precipitated morphine withdrawal on c-fos expression in rat corticotropin-releasing hormone neurons in the paraventricular hypothalamus and extended amygdala, Neuroscience letters, vol. 362, no. 1, pp. 39-43, doi: 10.1016/j.neulet.2004.02.033.

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Title Effect of naloxone-precipitated morphine withdrawal on c-fos expression in rat corticotropin-releasing hormone neurons in the paraventricular hypothalamus and extended amygdala
Formatted title Effect of naloxone-precipitated morphine withdrawal on c-fos expression in rat corticotropin-releasing hormone neurons in the paraventricular hypothalamus and extended amygdala
Author(s) Hamlin, A. S.
Buller, K. M.
Day, T. A.
Osborne, P. B.
Journal name Neuroscience letters
Volume number 362
Issue number 1
Start page 39
End page 43
Total pages 5
Publisher Elsevier Ireland
Place of publication Limerick, Ireland
Publication date 2004-05-13
ISSN 0304-3940
1872-7972
Keyword(s) morphine withdrawal
c-fos
corticotropin-releasing hormone
paraventricular hypothalamus
amygdala
immunohistochemistry
Summary Morphine withdrawal is characterized by physical symptoms and a negative affective state. The 41 amino acid polypeptide corticotropin-releasing hormone (CRH) is hypothesized to mediate, in part, both the negative affective state and the physical withdrawal syndrome. Here, by means of dual-immunohistochemical methodology, we examined the co-expression of the c-Fos protein and CRH following naloxone-precipitated morphine withdrawal. Rats were treated with slow-release morphine 50 mg/kg (subcutaneous, s.c.) or vehicle every 48 h for 5 days, then withdrawn with naloxone 5 mg/kg (s.c.) or saline 48 h after the final morphine injection. Two hours after withdrawal rats were perfused transcardially and their brains were removed and processed for immunohistochemistry. We found that naloxone-precipitated withdrawal of morphine-dependent rats increased c-Fos immunoreactivity (IR) in CRH positive neurons in the paraventricular hypothalamus. Withdrawal of morphine-dependent rats also increased c-Fos-IR in the central amygdala and bed nucleus of the stria terminalis, however these were in CRH negative neurons.
Language eng
DOI 10.1016/j.neulet.2004.02.033
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2004, Elsevier Ireland
Persistent URL http://hdl.handle.net/10536/DRO/DU:30044492

Document type: Journal Article
Collection: Faculty of Science, Engineering and Built Environment
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