Systemic administration of interleukin-1β activates select populations of central amygdala afferents

Buller, Kathryn M. and Day, Trevor A. 2002, Systemic administration of interleukin-1β activates select populations of central amygdala afferents, Journal of comparative neurology, vol. 452, no. 3, pp. 288-296, doi: 10.1002/cne.10389.

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Title Systemic administration of interleukin-1β activates select populations of central amygdala afferents
Author(s) Buller, Kathryn M.
Day, Trevor A.
Journal name Journal of comparative neurology
Volume number 452
Issue number 3
Start page 288
End page 296
Total pages 9
Publisher Wiley
Place of publication London, England
Publication date 2002-10-21
ISSN 0021-9967
Keyword(s) basolateral amygdala
basomedial amygdala
cholera toxin b-subunit
parabrachial nucleus
paraventricular thalamus
Summary The central nucleus of the amygdala (CeA) is activated robustly by an immune challenge such as the systemic administration of the proinflammatory cytokine interleukin-1β (IL-1β). Because IL-1β is not believed to cross the blood-brain barrier in any significant amount, it is likely that IL-1β elicits CeA cell recruitment by means of activation of afferents to the CeA. However, although many studies have investigated the origins of afferent inputs to the CeA, we do not know which of these also respond to IL-1β. Therefore, to identify candidate neurons responsible for the recruitment of CeA cells by an immune challenge, we iontophoretically deposited a retrograde tracer, cholera toxin b-subunit (CTb), into the CeA of rats 7 days before systemic delivery of IL-1β (1 μg/kg, i.a.). By using combined immunohistochemistry, we then quantified the number of Fos-positive CTb cells in six major regions known to innervate the CeA. These included the medial prefrontal cortex, paraventricular thalamus (PVT), ventral tegmental area, parabrachial nucleus (PB), nucleus tractus solitarius, and ventrolateral medulla. Our results show that after deposit of CTb into the CeA, the majority of double-labeled cells were located in the PB and the PVT, suggesting that CeA cell activation by systemic IL-1β is likely to arise predominantly from cell bodies located in these regions. These findings may have significant implications in determining the central pathways involved in generating acute central responses to a systemic immune challenge.
Language eng
DOI 10.1002/cne.10389
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2002, Wiley-Liss
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Document type: Journal Article
Collection: Faculty of Science, Engineering and Built Environment
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