Adrenaline and glycogenolysis in skeletal muscle during exercise : a study in adrenalectomised humans

Kjær, M., Howlett, K., Langfort, J., Zimmerman-Belsing, T., Lorentsen, J., B‡ülow, J., Ihlemann, J., Feldt-Rasmussen, U. and Galbo, H. 2000, Adrenaline and glycogenolysis in skeletal muscle during exercise : a study in adrenalectomised humans, Journal of physiology, vol. 528, no. 2, pp. 371-378.

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Title Adrenaline and glycogenolysis in skeletal muscle during exercise : a study in adrenalectomised humans
Author(s) Kjær, M.
Howlett, K.
Langfort, J.
Zimmerman-Belsing, T.
Lorentsen, J.
B‡ülow, J.
Ihlemann, J.
Feldt-Rasmussen, U.
Galbo, H.
Journal name Journal of physiology
Volume number 528
Issue number 2
Start page 371
End page 378
Total pages 8
Publisher Wiley-Blackwell Publishing
Place of publication Oxford, England
Publication date 2000-10
ISSN 0022-3751
1469-7793
Keyword(s) adrenaline
glycogenolysis
skeletal muscle
exercise
adrenalectomised humans
Summary 1. The role of adrenaline in regulating muscle glycogenolysis and hormonesensitive lipase (HSL) activity during exercise was examined in six adrenalinedeficient bilaterally adrenalectomised, adrenocorticohormonalsubstituted humans (Adr) and in six healthy control individuals (Con).

2. Subjects cycled for 45 min at •70% maximal pulmonary Oμ uptake (ýO2,max) followed by 15 min at •86% ýO2,max either without (−Adr and Con) or with (+Adr) adrenaline infusion that elevated plasma adrenaline levels (45 min, 4·49 ± 0·69 nmol l¢; 60 min, 12·41 ± 1·80 nmol l¢). Muscle samples were obtained at 0, 45 and 60 min of exercise.

3. In −Adr and Con, muscle glycogen was similar at rest (−Adr, 409 ± 19 mmol (kg dry wt)¢; Con, 453 ± 24 mmol (kg dry wt)¢) and following exercise (−Adr, 237 ± 52 mmol (kg dry wt)¢; Con, 227 ± 50 mmol (kg dry wt)¢). Muscle lactate, glucose6phosphate and glucose were similar in −Adr and Con, whereas glycogen phosphorylase (aÏa + b ² 100 %) and HSL (% phosphorylated) activities increased during exercise in Con only. Adrenaline infusion increased activities of phosphorylase and HSL as well as blood lactate concentrations compared with those in −Adr, but did not enhance glycogen breakdown (+Adr, glycogen following exercise: 274 ± 55 mmol (kg dry wt)¢) in contracting muscle.

4. The present findings demonstrate that during exercise muscle glycogenolysis can occur in the absence of adrenaline, and that adrenaline does not enhance muscle glycogenolysis in exercising adrenalectomised subjects. Although adrenaline increases the glycogen phosphorylase activity it is not essential for glycogen breakdown in contracting muscle. Finally, a novel finding is that the activity of HSL in human muscle is increased in exercising man and this is due, at least partly, to stimulation by adrenaline.
Language eng
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30045224

Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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