IEF pattern classification-derived criteria for the identification of epoetin-delta in urine

Lamon, Severine, Boccard, Julien, Sottas, Pierre-Edouard, Glatz, Nicolas, Wuerzner, Gregoire, Robinson, Neil and Saugy, Martial 2010, IEF pattern classification-derived criteria for the identification of epoetin-delta in urine, Electrophoresis, vol. 31, no. 12, pp. 1918-1924, doi: 10.1002/elps.200900645.

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Title IEF pattern classification-derived criteria for the identification of epoetin-delta in urine
Author(s) Lamon, SeverineORCID iD for Lamon, Severine
Boccard, Julien
Sottas, Pierre-Edouard
Glatz, Nicolas
Wuerzner, Gregoire
Robinson, Neil
Saugy, Martial
Journal name Electrophoresis
Volume number 31
Issue number 12
Start page 1918
End page 1924
Total pages 7
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
Place of publication Weinheim, Germany
Publication date 2010-06
ISSN 0173-0835
Keyword(s) discriminant analysis
Summary Epoetin-δ (Dynepo™ Shire Pharmaceuticals, Basing stoke, UK) is a synthetic form of erythropoietin (EPO) whose resemblance with endogenous EPO makes it hard to identify using the classical identification criteria. Urine samples collected from six healthy volunteers treated with epoetin-δ injections and from a control population were immuno-purified and analyzed with the usual IEF method. On the basis of the EPO profiles integration, a linear multivariate model was computed for discriminant analysis. For each sample, a pattern classification algorithm returned a bands distribution and intensity score (bands intensity score) saying how representative this sample is of one of the two classes, positive or negative. Effort profiles were also integrated in the model. The method yielded a good sensitivity versus specificity relation and was used to determine the detection window of the molecule following multiple injections. The bands intensity score, which can be generalized to epoetin-α and epoetin-β, is proposed as an alternative criterion and a supplementary evidence for the identification of EPO abuse.
Language eng
DOI 10.1002/elps.200900645
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
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Document type: Journal Article
Collection: School of Exercise and Nutrition Sciences
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