A short-term n-3 DPA supplementation study in humans

Miller, Eliza, Kaur, Gunveen, Larsen, Amy, Loh, Su Peng, Linderborg, Kaisa, Weisinger, Harrison S., Turchini, Giovanni M., Cameron-Smith, David and Sinclair, Andrew J. 2013, A short-term n-3 DPA supplementation study in humans, European journal of nutrition, vol. 52, no. 3, pp. 895-904.

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Title A short-term n-3 DPA supplementation study in humans
Author(s) Miller, Eliza
Kaur, Gunveen
Larsen, Amy
Loh, Su Peng
Linderborg, Kaisa
Weisinger, Harrison S.
Turchini, Giovanni M.
Cameron-Smith, David
Sinclair, Andrew J.
Journal name European journal of nutrition
Volume number 52
Issue number 3
Start page 895
End page 904
Total pages 10
Publisher Springer Medizin
Place of publication Heidelberg, Germany
Publication date 2013-04
ISSN 1436-6207
1436-6215
Keyword(s) n-3 polyunsaturated fatty acids (PUFA)
docosapentaenoic acid (DPA)
eicosapentaenoic acid (EPA)
docosahexaenoic acid (DHA)
fatty acid metabolism
Summary Purpose Despite the detailed knowledge of the absorption and incorporation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) into plasma lipids and red blood cells (RBC) in humans, very little is known about docosapentaenoic acid (DPA, 22:5 n-3). The aim of this study was to investigate the uptake and incorporation of pure DPA and EPA into human plasma and RBC lipids.

Methods Ten female participants received 8 g of pure DPA or pure EPA in randomized crossover double-blinded manner over a 7-day period. The placebo treatment was olive oil. Blood samples were collected at days zero, four and seven, following which the plasma and RBC were separated and used for the analysis of fatty acids.

Results Supplementation with DPA significantly increased the proportions of DPA in the plasma phospholipids (PL) (by twofold) and triacylglycerol (TAG) fractions (by 2.3-fold, day 4). DPA supplementation also significantly increased the proportions of EPA in TAG (by 3.1-fold, day 4) and cholesterol ester (CE) fractions (by 2.0-fold, day 7) and of DHA in TAG fraction (by 3.1-fold, day 4). DPA proportions in RBC PL did not change following supplementation. Supplementation with EPA significantly increased the proportion of EPA in the plasma CE and PL fractions, (both by 2.7-fold, day 4 and day 7) and in the RBC PL (by 1.9-fold, day 4 and day 7). EPA supplementation did not alter the proportions of DPA or DHA in any lipid fraction. These results showed that within day 4 of supplementation, DPA and EPA demonstrated different and specific incorporation patterns.

Conclusion The results of this short-term study suggest that DPA may act as a reservoir of the major long-chain n-3 fatty acids (LC n-3 PUFA) in humans.
Language eng
Field of Research 111103 Nutritional Physiology
Socio Economic Objective 920411 Nutrition
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2012, Springer-Verlag
Persistent URL http://hdl.handle.net/10536/DRO/DU:30046905

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