Biofunctionalization of 3D nylon 6,6 scaffolds using a two-step surface modification

Nuhiji, Edin, Wong, Cynthia S., Sutti, Alessandra, Tong, Lin, Kirkland, Mark and Wang, Xungai 2012, Biofunctionalization of 3D nylon 6,6 scaffolds using a two-step surface modification, ACS applied materials and interfaces, vol. 4, no. 6, pp. 2912-2919.

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Title Biofunctionalization of 3D nylon 6,6 scaffolds using a two-step surface modification
Author(s) Nuhiji, Edin
Wong, Cynthia S.
Sutti, Alessandra
Tong, Lin
Kirkland, Mark
Wang, Xungai
Journal name ACS applied materials and interfaces
Volume number 4
Issue number 6
Start page 2912
End page 2919
Total pages 8
Publisher American chemical society
Place of publication Washington, D.C.
Publication date 2012-06
ISSN 1944-8244
1944-8252
Keyword(s) biofunctionalization
3D porous scaffolds
surface modification
silanes
vacuum plasma treatment
nylon
Summary Nylon is a relatively inert polymer. The ability to easily functionalize nylon with biomolecules will improve the utilization of nylon in biological systems. A potential use of the biofunctionalized nylon scaffolds is in devices for cell therapeutics that can specifically select cells present in small numbers, such as hematopoietic stem cells. This study developed a versatile and simple two-step technique combining oxygen plasma treatment with wet silanization to graft biomolecules onto nylon 6,6 3D porous scaffolds. Scaffolds that were exposed to oxygen plasma exhibited up to 13-fold increase in silane attachment ((3-mercaptopropyl)trimethoxysilane/(3-aminopropyl)trimethoxysilane) compared to untreated scaffolds. To address the limitation of nondestructive characterization of the surface chemistry of 3D scaffolds, fluorescent CdSe/ZnS nanoparticles were used as a reporting tool for -NH(2) functionalized surfaces. Scaffolds that were covalently bound with neutravidin protein remained stable in phosphate buffered saline up to four months. Functionality of the neutravidin-grafted scaffolds was demonstrated by the specific binding of CD4 cells to the scaffold via CD4-specific antibody. Ultimately, these neutravidin-functionalized 3D nylon scaffolds could be easily customized on demand utilizing a plethora of biotinylated biomolecules (antibodies, enzymes and proteins) to select for specific cell of interest. This technique can be extended to other applications, including the enhancement of cell-scaffold interactions.
Language eng
Field of Research 090301 Biomaterials
030499 Medicinal and Biomolecular Chemistry not elsewhere classified
Socio Economic Objective 970109 Expanding Knowledge in Engineering
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2012, American Chemical Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30046919

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